PGS Kit

Aneuploidy can now be detected via next-generation sequencing (NGS) using DNA from a single or multiple cells isolated from preimplantation embryos.

The Ion ReproSeq™ PGS View Kit on the Ion PGM™ System delivers the fastest and most affordable NGS workflow for reliable aneuploidy detection of all 24 chromosomes in preimplantation embryos. Click on the button below to get a quote for the complete Ion ReproSeq PGS View solution.

Request a quote

View and order all products in Ion ReproSeq PGS View Kit workflow
Download app note

PGS webinar
Detection of aneuploidy in a single cell using the Ion ReproSeq PGS Kit
Ion ReproSeq PGS Kit

Watch the on-demand webinar featuring Dr. Luis A. Alcaraz of Bioarray, Spain.

Download the application note to see how the aneuploidy results compared to aCGH data.

Download the flyer to discover data generated from a single cell using the Ion PGM System.

See what customers are saying

“We’re very fond of this kit. We gained tremendous time reduction and the workflow is so easy that I don’t think it needs to be automated. We were able to perform entire workflow in less than 8 hours”

Sebastian PUKSZTA
Invicta, Poland

Rapid on-demand aneuploidy detection right on your benchtop

Leveraging the speed of Ion Torrent NGS, the Ion ReproSeq PGS View Kit workflow enables detection of whole-chromosome and chromosome arm events in as little as 8 hours* from just 6 pg of DNA from a single or multiple cells.

The Ion PGM System is designed to deliver high-quality sequencing data for aneuploidy detection with a range of chip outputs that scale with your sample throughput needs. Combined with low reagent pricing, this throughput flexibility helps minimize the need to batch embryos to achieve low cost per sample, making PGS more practical and affordable for laboratories of any size. 

The Ion ReproSeq PGS View Kit offers:

  • On-demand aneuploidy analysis and minimized sample batching enabled by a cost-effective workflow that scales to your throughput needs
  • The fastest NGS-based workflow for PGS that allows you to go from cell to results in as little as 8 hours*
  • A simple end-to-end workflow from cell to data with the preconfigured Ion Reporter™ Aneuploidy Analysis module for easy and automatic data analysis
  • A single tube workflow that minimizes sample contamination or loss

 

Ion ReproSeq PGS View Kit workflow: Cell to data in a single tube experiment

Ion ReproSeq PGS View Kit, when used with the Ion PGM™ System, Ion OneTouch ES, and Ion PGM Enrichment Beads, provide all reagents and materials required to detect aneuploidies starting from single or multiple cells. The entire workflow takes as little as 8 hours* from cells to analyzed data.

1 Extract DNA and
Construct library
Ion Torrent™ library kits

Ion SingleSeq™ Kit (included in any Ion ReproSeq PGS View Kit)

DNA is extracted from cells isolated from preimplantation embryo and barcoded in 3.5 hours.

 
2 Prepare
template
Ion Chef™ System
  • Ion PGM™ Template IA 500 Kit (included in any Ion ReproSeq PGS View Kit)
  • Ion PGM™ Enrichment Beads
  • Ion OneTouch™ ES instrument

  • Template is prepared in a fast isothermal amplification and enriched in just 2 hours

     
    3 Run
    sequence
    Proton System
  • Ion PGM™ Hi-Q™ Sequencing Kit (included in any Ion ReproSeq PGS View Kit)
  • Ion PGM System

  • Following chip loading, sequencing is performed and completed on the Ion PGM System in just 2.5 hours for the Ion 314™ Chip or 4.5 hours for the Ion 318™ Chip

     
    4 Analyze
    data
    Ion Reporter Software
  • Torrent Suite™ Software
  • Ion Reporter™ Software

  • The resulting data are automatically transferred to Torrent Suite Software for primary analysis, and aneuploidy status is determined using the Ion Reporter Software

    Ion PGM System—high-quality, accessible sequencing

    The Ion PGM System is designed to offer affordable, high-quality NGS to laboratories performing PGS. The Ion PGM System is a reliable sequencing platform that combines simple sample preparation and data analysis solutions with flexible chip output for project flexibility.

    Request a quote for the Ion PGM System

    Simple and automated data analysis solution

    Torrent Suite Software coordinates all the experiment planning and data processing steps necessary to complete your targeted sequencing workflow. Its intuitive, web-based interface through the Torrent Browser enables users to plan, monitor, and view sequencing run results quickly and simply. Data generated on the Ion PGM System are transferred to the PGM Torrent Server for base calling and automatically uploaded to Ion Reporter Software for further analysis.

    Aneuploidy analysis workflow on Ion Reporter Software makes analyzing and reporting aneuploidy results simple and fast. This low-pass coverage analysis workflow processes samples by comparing to a built-in bioinformatics control baseline to deliver the DNA ploidy status of each sample. Users can visualize aneuploidy profiles using the customized IGV Karyotype View and create their interpretive report.

    Access the free web-based Ion Reporter Software

    Product literature

    * Turnaround time is 8 hours with the Ion 314™ Chip for up to 2 samples, or 10 hours with the Ion 318™ Chip for up to 24 samples.