Fast, accurate results with gold-standard, rapid sequencing technologies
Targeted sequencing is a rapid and cost-effective way to detect known and novel variants in selected sets of genes or genomic regions. Gene sequencing can be accomplished using several different DNA sequencing methods, depending on the scale. Highly targeted single-gene sequencing is accurate, fast, and affordable with Sanger sequencing. Fast and affordable sequencing of multiple genes and panels can be accomplished with the Ion PGM™ System combined with Ion AmpliSeq™ technology.
Creating Targets for DNA Sequencing
Targeted sequencing requires upfront selection and isolation of genes or regions of interest, typically by either PCR amplification or hybridization-based capture methods. For sequencing a small number of targeted regions, PCR amplification is used in conjunction with Sanger sequencing. For larger numbers of genes or regions, the Ion AmpliSeq™ gene panels offer the ability to easily target and sequencing hundreds of genes in one day on the Ion PGM™ System. For higher density target regions of up to ~60 MB, the Ion TargetSeq™ Enrichment System is a cost-effective, fully customizable in-solution capture method for the Ion PGM™ System..