Many scientists see mapping of the human methylome as a logical follow-up to the Human Genome Project. Methylation status of DNA varies with age, tissue type, and environmental interactions. A number of diseases such as cancer are marked by altered methylation.
Because the methylome is much more variable than the genome, methylation mapping is inherently more complex than genome sequencing. We offer several strategies to help streamline your research studies.
Next-generation sequencing platforms enable high-throughput sequencing, mapping, and counting of short DNA reads (tags). In combination with traditional methylation profiling strategies (bisulfite conversion, digestion with methylation-sensitive restriction enzymes, and antibody- or 5-methylcytosine binding protein–based purification), NGS allows for comprehensive, genome-wide mapping of methylation sites.
For targeted analysis of methylation for specific regions, the gold-standard technique is bisulfite conversion and DNA sequencing.
Three genome-wide methylation profiling strategies are available for the Ion GeneStudio S5 series.
With Ion Torrent focused next-generation sequencing (NGS) technology, get the speed, scalability and precision you need to spend more time finding answers and less time looking for them. Further your epigenetics research with our comprehensive NGS solution, which enables a complete, targeted 3-day* workflow for quantitative methylation analysis across multiple loci when combined with the bisulfite conversion method.