Ion S5 and S5 XL NGS Sequencing Systems

Adopting next-generation sequencing (NGS) in your lab is now simpler than ever. The Ion S5 and Ion S5 XL Systems enable the simplest targeted sequencing workflow for your lab with industry-leading speed and affordability. Select from four different chip types, including the new Ion 510 Chip, to sequence a throughput range from 2 M to 80 M reads per run using just one instrument. Simply choose the chip type that matches your throughput and application needs.

Different chip types can now be combined in a single Ion Chef run when using Ion 510 & Ion 520 & Ion 530 Kit-Chef  and Torrent Suite Software version 5.6 or higher, allowing more flexibility in the workflow.

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“That’s it? That’s all I have to do to set it up?”

 – Susana Häggqvist, Research Engineer, Uppsala Genome Center      View customer videos ›

The S is for ...

DNA to data with less than 45 minutes of hands-on time

With simple cartridge-loaded reagents and a straightforward user interface, the Ion S5 and Ion S5 XL Systems make NGS fast and easy—ideal for any cancer or inherited disease research lab.

When combined with Ion Torrent AmpliSeq technology for target selection, the Ion Torrent Chef System for automated library and template preparation, and Ion Reporter Software for automated variant annotation, targeted sequencing becomes simpler than ever.

“The Ion Chef and S5 workflow is as simple as using a coffee pod machine but with espresso quality coffee.”

-  Dr. Lesley Cheng, Postdoctoral researcher, Department of Biochemistry and Genetics, La Trobe University, Australia

Ion S5 targeted sequencing workflow
Total hands-on time: less than 45 minutes

1 Construct library
Ion Torrent™ library kits

Hands-on time: 15 minutes

Ion AmpliSeq Panels on the Ion Chef System

2 Prepare template
Ion Chef™ System

Hands-on time: 15 minutes

Ion Chef System

3 Run sequence
Ion S5 or Ion S5 XL Systems

Hands-on time: 15 minutes

Ion S5 or Ion S5 XL Systems

4 Analyze data
Torrent Suite Software and Ion Reporter™ Software

Hands-on time: N/A

Torrent Suite Software

Single sequencer.  Multiple applications.

Each Ion S5 and Ion S5 XL System currently supports four different chip types, so you can run multiple applications on a single sequencer. This flexibility also means you don't have to batch samples to achieve the optimum cost efficiency. Just choose the chip type that matches your specific throughput or application needs.

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Learn more about Ion S5 System applications and see available app notes ›

Because every hour counts.

A few hours could make all the difference in your quest for the right answer—the answer that could have the power to impact time-sensitive outcomes.

Get results in as little as 24 hours (with 2.5-4 hour sequencer run times) using the Ion S5 XL System—the fastest turnaround of any benchtop sequencer.  

Because every sample matters.

Researchers worldwide are using Ion AmpliSeq technology for successful library preparation from challenging sample types, such as formalin-fixed, paraffin-embedded (FFPE) tissue, retrospective samples from fine needle aspirates, and cell-free DNA (cfDNA) extracted from blood.

With automated library preparation of Ion AmpliSeq panels on the Ion Chef System and easy sequencing on the Ion S5 or Ion S5 XL Sequencers, you can use as little as 1 ng of input DNA or RNA for the research of highly complex disease pathways.

Learn more about Ion AmpliSeq technology ›

Go from DNA to variants in a few clicks

Whether you are new to NGS or an experienced user, Torrent Suite Software and Ion Reporter Software make it easy for you to get started with your sequencing runs.  Plan, monitor, track, and analyze your runs using Torrent Suite Software (pre-installed on the Torrent Server).  Integrate, annotate, and interpret variants using Ion Reporter Software (cloud or local options available based on your needs). 

Learn more about NGS data analysis ›

With more than 3,700 global sales, service, and technical support specialists available to assist you in person, by phone, or online, you can be confident that we will be there when and where you need us most.
In addition to flexible service plans to meet your unique needs, and online or hands-on training programs to get your site up and running quickly, you will also have the opportunity to connect with peers in the Ion Torrent next-generation sequencing (NGS) community through regular user group meetings held at over 30 cities around the world. With over 30,000 members, Ion Community is a web-based forum that allows Ion Torrent researchers to openly share methods and data, to both evaluate the technology and build on it.

Ordering information:

Product(s) SKU or landing page
Ion AmpliSeq Panels Visit Ion AmpliSeq Designer
Ion Chef System 4484177
Ion S5 System A27212
Ion S5 XL System A27214
Visistat reference component