A major challenge for microbiologists and virologists is the prediction of patterns of evolution and emergence of disease agents. RNA viruses like coronaviruses share the biological feature of high genetic variability, which causes them to appear as clouds of mutants. Coronavirus variants also emerge through antigenic shift within animal reservoirs, such as bats, snakes, pangolins.
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Ion Torrent targeted next-generation sequencing (NGS) enables a streamlined research workflow for complete genome sequencing and epidemiological studies of SARS-CoV-2 (the coronavirus responsible for COVID-19). Through the use of a set of highly specific, universal coronavirus primers in combination with a high-fidelity master mix, all genomic segments are amplified, sequenced, and analyzed in an automated workflow on the Ion Torrent Genexus Integrated Sequencer. This new NGS solution enables you to rapidly go from nucleic acid to variant report in under a single day with minimal hands-on time.
The Ion AmpliSeq SARS-CoV-2 Insight Research Assay consists of two pools with amplicons 125–275 bp in length for complete coverage of over 99% of the viral genome and variants.
Benefits of Ion AmpliSeq SARS-CoV-2 Insight Research Assay for the Genexus Integrated Sequencer include:
Workflow and performance of the Ion AmpliSeq SARS-CoV-2 Insight Research Assay on the Genexus Integrated Sequencer
Ion AmpliSeq SARS-CoV-2 Insight Research Assay - GX
This assay is compatible with the Genexus Integrated Sequencer, which performs library preparation, sequencing, analysis, and reporting in an automated sample-to-result workflow
For Research Use Only. Not for use in diagnostic procedures.