Ion AmpliSeq™ Ready-to-Use Panels
Ready-to-use Primer Pools to Fast-Track and Simplify Oncology and Disease Research
Ion AmpliSeq™ Panels are ready-to-use ultra-high multiplexed PCR primer pools designed for target selection of thousands of amplicons using as little as 10 ng of input DNA. Cancer researchers can choose between the Ion AmpliSeq™ Cancer Panel and the Ion AmpliSeq™ Comprehensive Cancer Panel to target oncogenes for semiconductor sequencing. And, for broad-coverage disease research queries, the Ion AmpliSeq™ Inherited Disease Panel allows target selection of genomic regions implicated in 100 different disease states.
The breakthrough low-input DNA requirement of the Ion AmpliSeq™ panels enables the practical interrogation of research samples containing as little as 10 ng of input DNA, including formalin-fixed, paraffin-embedded (FFPE) and fine needle biopsy (FNB) samples.
Simplify and improve your research with ready-to-use Ion AmpliSeq™ Panels:
Accelerated workflow—optimized primer pools eliminate the primer design step and allow you to move directly to sequencing
Easy library construction—each panel consist of only one to four tubes, which minimizes experimental errors and complexity
Fastest sequencing—go from genomic DNA to variants in 9.5 hours with only 90 minutes of sequencing run time
Unprecedented scale—Scale up with increasing number of primer pairs per pool (up to ~3,000 primers in a single tube), three different capacity Ion chips, and 32 possible molecular barcodes
Select from the following Ready-to-Use Panels:
|Ion AmpliSeq™ Cancer Panel||Ion AmpliSeq™ Comprehensive Cancer Panel*||Ion AmpliSeq™ Inherited Disease Panel*|
|Target regions||Targets "hotspot' regions of 46 oncogenes and tumor suppressor genes with emphasis on whole exon coverage of KRAS, BRAF, and EGRF genes||Targets all exons within ~400 oncogenes and tumor suppressor genes and is ideal for novel variant discovery||Targets all exons for ~300 genes related to ~100 genetic disorders.|
|Gene coverage||Hotspots in 46 genes||All exons in ~400 genes||All exons ~300 genes|
|Mutation coverage||739 COSMIC mutations in 604 loci||xxx||xxx|
|Primer pool size||190 primer pairs in 1 tube||~12,500 primer pairs in 4 tubes||~6,000 primer pairs in 2 tubes|
|Specification (sensitivity for known mutations)||Down to 5% allelic frequency for known mutations at >500x sequencing coverage||Down to 10% allelic frequency for known mutations at >100x sequencing coverage||50% allelic frequency for known mutations at >20x sequencing coverage|
|Target coverage when mapped to hG19||98% mapped bases on target||95% mapped bases on target||95% mapped bases on target|
|Target uniformity||>98% of amplicons will be within 0.2x of the mean depth of coverage||95% of amplicons will be within 0.2x of the mean depth of coverage||95% of amplicons will be within 0.2x of the mean depth of coverage|
|Ion chip for one DNA sample||Ion 314™ Chip||Ion 318™ Chip||Ion 316™ Chip|
|Read length||1x100, 1x200, and paired-end 100x100|
*Commercially available in Q2 2012. The content provided herein may relate to products that have not been officially released and is subject to change without notice.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.