Ready-to-use Primer Pools to Fast-Track and Simplify Oncology and Disease Research

Ion AmpliSeq™ Panels are ready-to-use ultra-high multiplexed PCR primer pools designed for target selection of thousands of amplicons using as little as 10 ng of input DNA. Cancer researchers can choose between the Ion AmpliSeq™ Cancer Panel and the Ion AmpliSeq™ Comprehensive Cancer Panel to target oncogenes for semiconductor sequencing. And, for broad-coverage disease research queries, the Ion AmpliSeq™ Inherited Disease Panel allows target selection of genomic regions implicated in 100 different disease states.

The breakthrough low-input DNA requirement of the Ion AmpliSeq™ panels enables the practical interrogation of research samples containing as little as 10 ng of input DNA, including formalin-fixed, paraffin-embedded (FFPE) and fine needle biopsy (FNB) samples.

Simplify and improve your research with ready-to-use Ion AmpliSeq™ Panels:

Accelerated workflow—optimized primer pools eliminate the primer design step and allow you to move directly to sequencing

Easy library construction—each panel consist of only one to four tubes, which minimizes experimental errors and complexity

Fastest sequencing—go from genomic DNA to variants in 9.5 hours with only 90 minutes of sequencing run time

Unprecedented scale—Scale up with increasing number of primer pairs per pool (up to ~3,000 primers in a single tube), three different capacity Ion chips, and 32 possible molecular barcodes

Select from the following Ready-to-Use Panels:

  Ion AmpliSeq™ Cancer Panel Ion AmpliSeq™ Comprehensive Cancer Panel* Ion AmpliSeq™ Inherited Disease Panel*
Target regions Targets "hotspot' regions of 46 oncogenes and tumor suppressor genes with emphasis on whole exon coverage of KRAS, BRAF, and EGRF genes Targets all exons within ~400 oncogenes and tumor suppressor genes and is ideal for novel variant discovery Targets all exons for ~300 genes related to ~100 genetic disorders.
Gene coverage Hotspots in 46 genes All exons in ~400 genes All exons ~300 genes
Mutation coverage 739 COSMIC mutations in 604 loci xxx xxx
Amplicon length 75–125bp 175–225bp 175–225bp
Primer pool size 190 primer pairs in 1 tube ~12,500 primer pairs in 4 tubes ~6,000 primer pairs in 2 tubes
Specification (sensitivity for known mutations) Down to 5% allelic frequency for known mutations at >500x sequencing coverage Down to 10% allelic frequency for known mutations at >100x sequencing coverage 50% allelic frequency for known mutations at >20x sequencing coverage
Target coverage when mapped to hG19 98% mapped bases on target 95% mapped bases on target 95% mapped bases on target
Target uniformity >98% of amplicons will be within 0.2x of the mean depth of coverage 95% of amplicons will be within 0.2x of the mean depth of coverage 95% of amplicons will be within 0.2x of the mean depth of coverage
Ion chip for one DNA sample Ion 314™ Chip Ion 318™ Chip Ion 316™ Chip
Barcode Compatible Yes
Read length 1x100, 1x200, and paired-end 100x100


*Commercially available in Q2 2012. The content provided herein may relate to products that have not been officially released and is subject to change without notice.

Support

Please contact Ion Torrent™ at:
1-87-SEQUENCE (1-877-378-3623)
Email the Support Team >

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

Support

NEW   Next-Generation Sequencing Support Center
Find tips, troubleshooting help, and resources for your next-gen sequencing applications.