Ion Chef System
Simple, reproducible automated sequencing
The Ion Torrent Ion Chef System simplifies the workflow for Ion S5, Ion PGM, and Ion Proton systems, providing an automated, walk-away workflow solution for Ion AmpliSeq library preparation and reproducible template preparation and chip loading.
Simplified workflow helps save time and labor
- Intuitive instrument set-up for all automated workflows
- Increased lab productivity—just 15 minutes of set-up time
Reduced run variability
- Reduced user intervention enables reproducible chip loading for users of any experience level
- Sample and reagent tracking with onboard tracking system
Ion AmpliSeq library preparation automation
- Expanded Ion Chef System capabilities enable automation of Ion AmpliSeq library generation
- Accommodates one- or two-pool Ion AmpliSeq research panels for DNA analysis
Ion AmpliSeq library preparation automation with the Ion Chef System
Free up your time for higher-value activities through the automation of your Ion AmpliSeq targeted sequencing workflow with the Ion Chef System. Fifteen minutes of up-front hands-on time using pre-packaged ready-to-go library preparation reagents enables walk-away convenience—return 7 hours later to take equalized and pooled libraries on to the template preparation step. The automated workflow supports Ion AmpliSeq one- and two-pool designs.
- Load reagents & consumables
- Set run parameters
- Load up to 8 DNA samples & Ion AmpliSeq panel
- Targeted PCR amplification
- Digest primers
- Ligate library adaptors
- Equalize & pool libraries
- Load reagents & consumables
- Load library samples
- Set run parameters
- Library amplification
- ISP recovery & enrichment
- Chip loading
Automated template preparation & chip loading with the Ion Chef System
The Ion Chef System simplifies the Ion Torrent next-generation sequencing workflow by integrating automated template preparation and chip loading in a single instrument for maximum efficiency and throughput. Simply select the run parameters using the intuitive touch screen interface, load the library samples, reagents, and consumables, and go.
Simplifying Next-Gen Sequencing Template Prep with Ion Chef
Ion Chef enables walk-away freedom
Ion Chef System templating and chip loading workflow
- Load reagents & consumables
- Load library samples
- Set run parameters
- Library amplification
- ISP recovery & enrichment
- Chip loading
Run sequence on either:
- Ion S5 System
- Ion PGM System or
- Ion Proton System
The Ion Chef System simplifies the next-generation sequencing workflow by integrating automated template preparation and chip loading in a single instrument for maximum efficiency and throughput. Simply select the run parameters using the intuitive touch screen interface, load the library samples, reagents, and consumables, and go.
Compatible with the Ion S5, Ion PGM, and Ion Proton systems, the Ion Chef System supports all Ion chip types, providing flexibility for any project type and size, including use of Ion AmpliSeq libraries for targeted DNA or RNA sequencing.
The Ion Chef System supports:
Instrument |
Ion Chip |
Read Length |
Key Research Applications |
Ion S5 System |
Ion 510™ Chip |
Up to 400 bp |
Targeted DNA sequencing, targeted RNA sequencing, small RNA sequencing, de novo microbial sequencing, bacterial typing, viral typing, metagenomics, sequencing by genotyping |
Ion 520™ Chip |
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Ion 530™ Chip |
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Ion 540™ Chip |
Up to 200 bp | Exome sequencing, transcriptome sequencing, copy number analysis | |
Ion PGM System |
Ion 314™ Chip |
Up to 400 bp |
Targeted DNA sequencing, targeted RNA sequencing, small RNA sequencing, de novo microbial sequencing, bacterial typing, viral typing, metagenomics, sequencing by genotyping |
Ion 316™ Chip |
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Ion 318™ Chip |
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Ion Proton System |
Ion PI™ Chip |
Up to 200 bp |
Exome sequencing, transcriptome sequencing, copy number analysis |
For Research Use Only. Not for use in diagnostic procedures.