Simple, reproducible automated
sequencing

The Ion Torrent™ Ion Chef™ System simplifies the workflow for Ion S5™, Ion PGM™, and Ion Proton™ systems, providing an automated, walk-away workflow solution for Ion AmpliSeq™ library preparation and reproducible template preparation and chip loading.

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Simplified workflow helps save time and labor

  • Intuitive instrument set-up for all automated workflows
  • Increased lab productivity—just 15 minutes of set-up time

Reduced run variability

  • Reduced user intervention enables reproducible chip loading for users of any experience level
  • Sample and reagent tracking with onboard tracking system

Ion AmpliSeq library preparation automation

  • Expanded Ion Chef System capabilities enable automation of Ion AmpliSeq library generation
  • Accommodates one- or two-pool Ion AmpliSeq research panels for DNA analysis
Ion AmpliSeq library preparation automation with the Ion Chef System

Free up your time for higher-value activities through the automation of your Ion AmpliSeq™ targeted sequencing workflow with the Ion Chef System.  Fifteen minutes of up-front hands-on time using pre-packaged ready-to-go library preparation reagents enables walk-away convenience—return 7 hours later to take equalized and pooled libraries on to the template preparation step. The automated workflow supports Ion AmpliSeq one- and two-pool designs.

Dr. Mark Andersen from Thermo Fisher R&D discusses the capabilities and benefits of using the Ion Chef System for Ion AmpliSeq automated library preparation. View now

See how easy it is to automate Ion AmpliSeq library preparation on the Ion Chef System. View now

1 Preparation


Ion Chef

  • Load reagents & consumables
  • Set run parameters
  • Load up to 8 DNA samples & Ion AmpliSeq panel
2 Automated Ion AmpliSeq library preparation
Ion Chef System

  • Targeted PCR amplification
  • Digest primers
  • Ligate library adaptors
  • Equalize & pool libraries

3 Preparation


Ion Chef

  • Load reagents & consumables
  • Load library samples
  • Set run parameters

4 Automated template prep & chip loading
Ion Chef System

  • Library amplification
  • ISP recovery & enrichment
  • Chip loading
Automated template preparation & chip loading with the Ion Chef System

The Ion Chef System simplifies the Ion Torrent next-generation sequencing workflow by integrating automated template preparation and chip loading in a single instrument for maximum efficiency and throughput. Simply select the run parameters using the intuitive touch screen interface, load the library samples, reagents, and consumables, and go. 

See how the Ion Chef System helps simplify your sequencing workflow. View now

AIBio Tech increases workflow efficiency with the Ion Chef System. Watch the #LabChat video and learn how

Ion Chef System templating and chip loading workflow

1 Preparation


Ion Chef

  • Load reagents & consumables
  • Load library samples
  • Set run parameters
2 Automated template preparation and chip loading
Ion Chef System

  • Library amplification
  • ISP recovery & enrichment
  • Chip loading

3 Sequencing


Ion PGM, Ion S5

Run sequence on either:

  • Ion S5 System
  • Ion PGM System or
  • Ion Proton System

The Ion Chef System simplifies the next-generation sequencing workflow by integrating automated template preparation and chip loading in a single instrument for maximum efficiency and throughput. Simply select the run parameters using the intuitive touch screen interface, load the library samples, reagents, and consumables, and go.

Compatible with the Ion S5, Ion PGM, and Ion Proton systems, the Ion Chef System supports all Ion chip types, providing flexibility for any project type and size, including use of Ion AmpliSeq libraries for targeted DNA or RNA sequencing.

The Ion Chef System supports:

Instrument

Ion Chip

Read Length

Key Research Applications

Ion S5 System

Ion 520™ Chip

Up to 400 bp

Targeted DNA sequencing, targeted RNA sequencing, small RNA sequencing, de novo microbial sequencing, bacterial typing, viral typing, metagenomics, sequencing by genotyping

Ion 530™ Chip

Ion 540™ Chip

Up to 200 bp Exome sequencing, transcriptome sequencing, copy number analysis

Ion PGM System

Ion 314™ Chip

Up to 400 bp

Targeted DNA sequencing, targeted RNA sequencing, small RNA sequencing, de novo microbial sequencing, bacterial typing, viral typing, metagenomics, sequencing by genotyping

Ion 316™ Chip

Ion 318™ Chip

Ion Proton System

Ion PI™ Chip

Up to 200 bp

Exome sequencing, transcriptome sequencing, copy number analysis