Resequencing experiments are fundamental in basic and clinical research as scientists look for causative mutations within populations. Targeted resequencing involves the comparative analysis of candidate genes or regions and requires a high level of accuracy to identify low frequency SNPs and structural variants.  A large number of genes must be analyzed to elucidate such variations, making next generation sequencing an attractive solution.

Step-by-Step Guide to Targeted Resequencing Sample Prep Products

All Next Generation Sequencing Platforms


PureLink™ Genomic DNA Mini Kit
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.

All Next Generation Sequencing Platforms


SequalPrep™ System
Robust target enrichment by long PCR (5-20kb) for enhanced sequencing of targeted regions, followed by one-step amplicon purification and input equalization in a convenient plate for accurate target representation.

Cot-1DNA® Blocking Reagent
Increase hybridization sensitivity on array-based exon capture platforms by blocking repetitive sequences.

SOLiD® Applications


SOLiD® Fragment Library Construction
Fully optimized complete set of reagents, columns, and size selection gels to generate fragment libraries for use on SOLiD® System.

SOLiD® Mate-Paired Library Construction
Fully optimized, validated, complete set of reagents to construct mate-paired libraries for use on SOLiD® System.

All Next Generation Sequencing Platforms


E-Gel™ SizeSelect™ Gels
Rapid size separation and recovery system for library construction.

SOLiD® Applications


SOLiD® Library TaqMan® Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD® library for optimal input in emulsion PCR (ePCR).

SOLiD® Applications


SOLiD® Fragment Library Barcoding Kits
Unique barcode sequences for SOLiD® fragment libraries to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

Support

NEW   Next-Generation Sequencing Support Center
Find tips, troubleshooting help, and resources for your next-gen sequencing applications.