Whether you are new to next-generation sequencing (NGS) or want to learn about the various applications of NGS, we have the learning materials to help you understand the technology and get started quickly.
NGS is a high-throughput method achieved by sequencing clonally amplified DNA templates on a massively parallel scale. The speed, throughput, and accuracy achieved with NGS has revolutionized genetic science and enabled applications once thought of as science fiction.
A general overview of the basics of NGS and the workflow from library construction to data analysis.
Learn about the different concepts of sequencing coverage and how changes in coverage can occur due to variations in genetic sequence. Understand why coverage affects variant discovery and how throughput is related to coverage.
Understand the advantages and disadvantages of general sequencing methods used with NGS, from whole genome sequencing to exome and targeted sequencing.
RNA sequencing (RNA-Seq) leverages the advantages of NGS to detect and quantify RNA in a biological sample at a given point in time. This article goes over basic experimental considerations and the workflow for various RNA-Seq methods.
This article provides further details on targeted sequencing approaches. Learn how Ion AmpliSeq technology enables numerous applications with a rapid, simple, and highly multiplexed amplicon-based enrichment solution.
For Research Use Only. Not for use in diagnostic procedures.