• Enables fast and accurate quantitation of gene expression linked to self-renewal and cell differentiation
  • Lot-to-lot consistency
  • Offered in multiple pack sizes: 500 mL, 100 mL and the One Shot™ 50 mL bottle
  • Superior quantitative, reproducible results compared to older assay techniques
  • Only GIBCO® offers you ES Cell Qualified FBS with this industry-leading assay!

Qualified for Performance: New qRT-PCR Assay

GIBCO® ES Cell Qualified FBS uses an industry-leading qualification assay which includes state-of-the-art instrumentation, cell culture, and assay design. The improved assay challenges the FBS to encourage self-renewal under stringent conditions without other methods to prevent differentiation, including feeder cell growth or Leukemia Inhibitory Factor. While the old assay relied on technician expertise and identification of colony types by cell-surface markers, the new quantitative PCR-based method provides a numerical output of the level of expression of genes of interest for measurement.

Improved Pluripotency

The pluripotency gene tested is a transcription factor that is essential to maintain self-renewal of undifferentiated embryonic stem cells. Studies have shown that the essential function of this gene is to stabilize ES cells in a pluripotent state by maintaining the requisite level of expression of other key transcription factors.

Reduced Differentiation

The differentiation gene is involved in various functions of development and differentiation, including trophoblast development and mesoderm formation, which are among the first indications of differentiation.