Embryonic stem cells are derived from the inner cell mass of blastocysts and are used in a variety of different research studies regarding biological functioning. Gibco Embryonic Stem (ES) Cell FBS Qualified uses an industry-leading qualification assay, which includes state-of-the-art instrumentation, cell culture, and assay design. The improved assay was developed by our Stem Cell R&D and Quality teams to confirm the ability of our FBS to achieve high plating efficiencies and maintain pluripotency of ES cells. The new method evaluates the ability of FBS to support plating, pluripotency and proliferation in mESCs by quantifying pluripotency marker expressing colonies in combination with morphological evaluation.
When performing research on stem cells, researchers need cells that can support their testing methods. It is critical that the stem cells maintain the ability to differentiate into the cell type of interest in order to test expression and other characteristics. Whether researching vitro fertilization or the relationship between cancer and mutation in human embryos, there are many types of ES cells that can be used in various methods to help researchers conduct their studies.
Advantages of using Gibco Embryonic Stem Cell FBS Qualified
- Specially tested for the ability to sustain undifferentiated ES cells (Figure 1A) while maintaining karyotype integrity (Figure 2), LIF responsiveness (Figure 3) and pluripotency markers (Figure 1B)
- New improved screening with germ line–competent PRX129/X1 mESC line using a predictive assay that measures plating efficiency and pluripotency maintenance
- High consistency between lots with proven applications in iPSC generation and PSC culture
- Now available in the aliquot-free One Shot 50 mL bottle designed specifically for FBS use
Ideally for researchers studying…*
- Hormones or hormone receptors (androgens, estrogens, progesterone)
- Induced pluripotent stem cells (iPSCs)
- Cellular reprogramming
- Embryonic stem cells (ESCs)
- Embryonic development
Using ES cells to study disease, cancer, or cellular mutation can help pave the way for new treatment options and potential biological advancements. To learn more about the different types of stem cells available, methods of research and details on protocol, call the professionals at Thermo Fisher today.
mESCs grown in ES Cell FBS Qualified exhibit pluripotency traits
mESCs grown in media supplemented with ES Cell FBS Qualified exhibit morphological characteristics of pluripotency: well-defined refractive edges, tight colonies with small cells, and few differentiated cells (Figure 1A). ESC colonies grown in ES Cell FBS Qualified also stain positive for alkaline phosphatase (AP). AP is a pluripotency marker expressed by PSCs.
Figure 1. Image analysis of mESCs colonies grown in media containing Gibco ES Cell FBS Qualified. (A) Phase-contrast image of mESC colonies grown in media containing ES Cell FBS Qualified showing colonies with morphological traits consistent with pluripotency. (B) An overlay of the fluorescence image (obtained after staining the sample with Alkaline Phosphatase (AP) Live Stain) with the phase-contrast image of mESC colonies, showing uniform AP staining. Colonies that appear green are expressing AP and are therefore pluripotent.
Figure 2. mESCs grown in media supplemented with ES Cell FBS Qualified exhibit normal karyotypes. Normal karyotype report for PRX129/X1 mESC line. 18 of 20 cells tested exhibited a normal karyotype. (Cell Line Genetics).
Figure 3. mESCs cultured in media supplemented with ES Cell FBS Qualified maintain LIF responsiveness. When LIF is removed from mESC media, normal mESC cultures respond with widespread differentiation. PRX129/X1 murine ES cells grown with and without LIF demonstrate this response.
ES Cell FBS Qualified is able to support reprogramming workflows
Figure 4. When used to supplement growth media, ES Cell FBS Qualified is able to support generation of iPSCs from both mouse and human fibroblasts. In a standard workflow using Sendai virus–based reprogramming (CytoTune iPS 2.0 Sendai Reprogramming Kit), both murine and human fibroblasts successfully generated iPSCs.
Rigorous screening methodology helps ensure superior FBS performance
Each lot of ES Cell FBS Qualified is screened using PRX129/X1 murine ESC line using an assay that measures plating efficiency, morphology (Figure 5), and stem cell marker expression (Figure 6).
Figure 5. Plating efficiency and colony morphology assessed in murine embryonic stem cells grown in media containing ES Cell Qualified FBS. (A) Undifferentiated colonies (top row) appear bright and sharp, and differentiated colonies (bottom row) appear dim, fuzzy and flattened when stained with alkaline phosphatase. (B) Methylene blue stains all cells and is used to count the total colony number. (C) Results plotted as % AP+/MB+ demonstrates assay consistency among three users.
ES Cell FBS Qualified supports pluripotency in mESCs
Figure 6. Phase contrast and fluorescent images of mESC colonies. mESCs cultured in media supplemented with ES Cell Qualified FBS exhibit the morphological traits of well-defined refractive edges, tight colonies with small cells, and few differentiated cells. These cells also exhibit pluripotency via Alkaline Phosphatase (AP) Live Stain and the expression of Oct4, and Sox2.
- Paola Rebuzzini et. al (2020). Chronic cypermethrin exposure alters mouse embryonic stem cells growth kinetics, induces Phase II detoxification response and affects pluripotency and differentiation gene expression.
- Minsuh Kim et. al (2019). Patient derived lung cancer organoids as in vitro cancer models for therapeutic screening.
- Neda Eskandari et. al (2018). The combination of retinoic acid and estrogen can increase germ cells gene expression in mouse embryonic stem cells derived primordial germ cells.
- Rong Hu et. al (2017). Transplantation of donor-origin mouse embryonic stem cell-derived thymic epithelial progenitors prevents the development of chronic graft-versus-host disease in mice.
- Dasa Bohaciakova et. al (2017). An efficient method of generation of knockout human embryonic stem cells using CRISPR/Cas9 system.
- Stephen P Jenkinson et. al (2017). Embryonic stem cell-derived neurons grown on multi-electrode arrays as a novel in-vitro bioassay for the detection of Clostridium botulinum neurotoxins.
* These results are based on a review of approximately 10,000 publications using query terms based on the six specialty FBS products offered by Thermo Fisher Scientific. These terms were generated by the MeSH (medical subject headings) taxonomy based on the full text of the paper.
For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.