1.  How can we find the protocol for these cells?

Protocols can be found in the manual provided with the cells

2.  Do I need to use MSC-Qualified FBS?

For optimal cell expansion, we recommend MSC-Qualified FBS.  If you are interested in trying any other FBS, we recommend using MSC-Qualified FBS as a control in your evaluation.

3.  Can we use MesenPro RS as a culture media?

The cells expand quite well in MesenPro.  However for optimal growth performance, we recommend culturing and expanding the cells in alpha-MEM (SKU# 32571-036) and MSC-Qualifed FBS (SKU # 12662-011).

4.  What is the best way to detect alkaline phosphatase activity?

For easy detection of alkaline phosphatase activity, Invitrogen™ offers the ELF® 97 Endogenous Phosphatase Kit (SKU# E-6601).  The kit includes the ELF® 97 phosphatase substrate that upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity.  This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift, 180 nm that makes it easily distinguishable from endogenous fluorescence.

5.  How many passages can the cells be expanded?

We have tested the cells to expand up to 3 passages.  But it is possible that the cells can go beyond 3 passages.

6.  Do all cells adhere when the cells are thawed?

A small % of cells might not adhere when the cells are first thawed.  However if the adherent cells are expanded, the expanded cells will be adherent in future passages.

7.  What is the strain of rat used in this product?

StemPro Rat Alk Phos-expressing MSCs are isolated from the bone marrow of pooled young transgenic Fisher 344 rats ≤ 8 weeks of gestation.

8.  Can we use these cells in animal transplant experiments?

Invitrogen™ has not performed experiments to demonstrate the capability.  However, we recommend that customers evaluate the product for their experiments.