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NSC media and reagents are designed to help minimize adaptation time, while maximizing cell growth and function during neural stem cell maintenance, induction, and expansion. Explore these high quality Gibco products for NSC culture organized by culture stage.
| Culture stage | Medium/Supplement | Growth factors | Substrate/Coating | Notes |
|---|---|---|---|---|
| PSC maintenance (hESC/hiPSC) | Essential 8 Medium | – | Vitronectin rhLaminin-521 | Defined, xeno-free, and feeder-free medium for growth and expansion of human PSCs. |
| PSC expansion/maintenance | StemFlex Medium | – | Vitronectin Geltrex Flex matrix rhLaminin-521 | Robust feeder-free PSC culture optimized for gene editing and offers flexibility in feeding schedules and culture conditions. |
| Induction (PSC → NSC) | PSC Neural Induction | – | Geltrex Flex matrix | Supports efficient induction of human PSCs to NSCs in ~7 days, without requiring EB formation. |
| NSC expansion/maintenance | StemPro NSC SFM Kit | Includes:
| CELLstart substrate Geltrex Flex matrix Laminin | Kit contains all reagents for complete, serum-free NSC expansion medium. Supports adherent and neurosphere culture. |
| NSC expansion/maintenance | StemPro Neural Supplement | CELLstart substrate Geltrex Flex matrix Laminin | Supplement for creating your own serum-free NSC expansion/maintenance media. See StemPro data. | |
| Differentiation (NSC → neurons, glia) | Neurobasal medium B-27 Supplement | None (withdraw bFGF/EGF) | Geltrex Flex matrix Laminin | Supports the differentiation of NSCs to a neuronal fate. |
| Differentiation (NSC → neurons) | CultureOne supplement | – | Laminin Poly-D-lysine | Can be added to Neurobasal media along with B-27 supplements to enhance neural differentiation, maturation, and functionality. |
| Neuron maturation | B-27 Plus Neuronal Culture System | – | Poly-D-Lysine | Robust culture system providing superior neuronal survival, increased neurite outgrowth and improved functionality |
Once your stem cells are differentiated into neurons or glia, keep them happy and healthy with Gibco products for neuronal cell culture:
Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.