• Fast
  • Requires less sample
  • Detect 4 early differentiation markers in one PCR reaction

Stem cell characterization made easy

Human Tri-lineage Multiplex PCR Kit:

  • Rapidly and reliably performs routine characterization of human embryonic stem cells (hESCs) and human embryonal carcinoma stem cells (hECs).
  • Detects markers for the undifferentiated state and all three embryonic lineages in one PCR reaction using a convenient multiplex RT-PCR assay.
  • Requires smaller sample volumes than traditional characterization methods.
  • Contains all the reagents necessary to quickly isolate RNA, make cDNA and perform PCR to detect the expression of differentiation markers.

Early Differentiation Markers

The STEMPRO® EZChek™ Kit contains primers to detect the following human genes in one multiplex PCR reaction:

  • Oct4/Pou5f1: marker for the pluripotent state of hESCs and hECs
  • AFP: endoderm lineage marker
  • ACTC1: mesoderm lineage marker
  • Sox1: ectoderm lineage marker
  • GAPDH: internal RNA standard for normalizing mRNA levels

Applications of the STEMPRO® EZChek™ Kit

This figure illustrates the characterization of BG01v human embryonic stem cells and a BG01v 21-day embryoid body (EB) control sample using the STEMPRO® EZChek™ Kit. BG01v human embryonic stem cells express only the pluripotency marker while in the BG01v EB control sample the expression of the pluripotency marker is down-regulated and the differentiation markers are induced.

Need a control for your characterization experiments?
Use the BG01V/hOG Cells Variant hESC hOct4-GFP Reporter Cells

Components of the STEMPRO® EZChek™ Kit

The Kit contains the following components:

  • PureLink™ Micro-to-Midi™ Total RNA Purification System: rapidly and reliably isolate high-quality total RNA from your hESCs or hECs.
  • SuperScript® III Reverse Transcriptase: provides high yields of first-strand cDNA in a convenient high-throughput supermix format.
  • STEMPRO® EZChek™ Human Tri-Lineage Primer Mix: contains primers for detecting five human genes in one multiplex PCR reaction.