Stem Cell Intel | Issue 3
Each quarter, Stem Cell Intel will provide you with the latest product news, a column from one of our experts, upcoming industry events, and easy access to technical tools such as publications, protocols, FAQs, and more. To receive Stem Cell Intel, please click the subscribe button below.
Interact with stem cell thought leaders at the 2nd annual 24 Hours of Stem Cells event running concurrently around the world for 24 hours on November 6. Registration is free.
Forget about applying for travel grants or finding someone to watch your cells while you’re away at a conference. In this free event, researchers from all over the world come together online to network with peers and learn about the newest research.
During this next-gen virtual conference you can:
- Log in and out at your convenience for on-demand content or view live webcasts.
- Enrich your perspectives of stem cell research in a global context.
- Submit an abstract by October 1 and gain global visibility for your research in our virtual poster forum.
Get all the benefits of attending an international scientific conference, without all of the hassles.
Unleash your stem cells with Lipofectamine® 3000 Transfection Reagent
Induced pluripotent stem cells (iPSCs) hold immense promise for the future of regenerative medicine and personalized therapeutic treatments for a myriad of diseases and conditions. Lipofectamine® 3000 Transfection Reagent was developed to unleash the power of stem cells by providing a highly efficient, cost-effective nucleic acid delivery alternative to electroporation. This advanced lipid nanoparticle technology minimizes the stress on cells caused by electroporation, simplifies the reprogramming workflow, and enables advanced gene-editing technologies.
Now improved! Gibco® Mouse (ICR) Inactivated Embryonic Fibroblasts
We recently improved Gibco® Mouse (ICR) Inactivated Embryonic Fibroblasts (Cat. No. A24903). Each vial isolated from mouse strain ICR provides over 2 million viable cells and >80% post-thaw viability. The mouse embryonic fibroblasts provide a feeder layer for culturing either mouse or human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs), and for pluripotent marker expression (Oct 4).
KnockOut™ Serum Replacement—the key to pluripotent stem cell culture
KnockOut™ Serum Replacement has been the key to feeder-based stem cell culture systems since 1997. KnockOut™ Serum Replacement is a defined, FBS-free supplement that can be used for the routine maintenance and derivation of human and non-human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). KnockOut™ Serum Replacement can be used throughout the entire PSC workflow—for reprogramming, culture, cryopreservation, recovery, and differentiation.
KnockOut™ Serum Replacement has been cited in over 2,300 publications since it was developed, and it continues to be an important part of the PSC workflow. With the ever-increasing number of iPSC lines being cultured, we recommend batch testing of KnockOut™ Serum Replacement to help ensure optimal performance.
Easy and efficient
Customers have been most excited to see the use of these kits during the reprogramming sequence. The kits allow staining of reprogrammed colonies at various time points during the process, providing confidence that the positively staining emerging colonies are on the right track. Another valuable feature of the kits is that, at the end of the reprogramming sequence, it is easy to choose positive colonies, pick and transfer each colony to a new well, and verify reprogramming success, as the colony still displays the fluorescent markers in the new well. The kits are also helpful in distinguishing between partially and fully reprogrammed cells.
Enable successful reprogramming of PBMCs to iPSCs
Human induced pluripotent stem cells (iPSCs) derived from adult somatic cells hold great promise for disease modeling and clinical therapies. Most iPSCs are derived from fibroblasts donated from skin biopsies and expanded prior to reprogramming. The ability to use blood from donors or sample banks of frozen peripheral blood mononuclear cells (PBMCs) is attractive, but reprogramming from peripheral blood sources has cytotoxicity and low reprogramming efficiency challenges. Our research goal was to enable successful and consistent generation of iPSCs from frozen PBMCs using the CytoTune®–iPS 2.0 Sendai Reprogramming Kit, by optimizing spinoculation, polybrene, cytokine, and other workflow conditions.
A snapshot from ISSCR 2014: thirteen posters covering reprogramming, characterization, and much more
The annual meeting of the International Society for Stem Cell Research always draws attention to the latest in stem cell research and technology. Our team of scientists presented a broad range of posters at this year’s event, showcasing new technology and stem cell workflow enhancements. The topics featured in the thirteen posters presented included:
- Increasing transfection efficiency with Lipofectamine® 3000 reagent, even in difficult stem cells
- Optimizing conditions for high-efficiency reprogramming with CytoTune® Sendai reprogramming kits
- Characterizing pluripotent stem cells, cardiomyocytes, and neural cells with tools and antibody-based markers
- Building stem cell–derived disease modeling systems using donor cells and genome-editing technology
Life Technologies Stem Cell Training Workshop
October 7–9, 2014
These workshops provide customers with hands-on training in techniques for culturing and characterizing human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), as well as reprogramming techniques for the creation of iPSCs. Whether you are new to pluripotent stem cell research or need a refresher course, our R&D scientists can provide detailed training so that you can feel confident using stem cells in your research.
Stem Cells Meeting on the Mesa
October 7–9, 2014
La Jolla, CA, USA
Mark your calendar now for this year's conference bringing together nearly 800 attendees from across the globe for regenerative medicine's premier annual conference. This meeting, attended by top regenerative medicine companies, big pharma, disease philanthropies, investors, and clinicians, helps to bridge academia and industry through one-on-one meetings, furthering the translation of clinical research.
NYSCF 9th Annual Translational Stem Cell Research Conference
October 22–23, 2014
New York, NY, USA
The NYSCF conference is unique: it focuses on translational stem cell research, demonstrating the potential to advance cures for the major diseases of our time. It is designed for all professionals with an interest in stem cell research, including physicians, researchers, clinical investigators, government and health officials, professors, postdoctoral fellows, and graduate students. The panels on Wednesday are designed to reach a broader audience, including the lay public.
2nd International Annual Conference of the GSCN
November 3–5, 2014
The German Stem Cell Network (GSCN) is a network association aimed at creating synergies between all areas of basic and applied stem cell research and providing an interface between science, politics, education, and society as a whole.
24 Hours of Stem Cells
November 6, 2014
The 2nd annual 24 Hours of Stem Cells event is coming soon. Forget about applying for travel grants or finding someone to watch your cells while you’re away at another conference. In this event, researchers come together online to network with peers and learn about the newest research from all over the world. Get all the benefits of attending a scientific conference, without the hassles of travel.
For Research Use Only. Not for use in diagnostic procedures.