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Product news

Single-cell passaging for PSCs

RevitaCell Supplement was developed for use with Essential 8 Medium to enable maximum cell viability when performing single-cell passaging of pluripotent stem cells (PSCs). RevitaCell Supplement includes a more specific ROCK inhibitor than traditional ROCK inhibitors, which is coupled with molecules containing antioxidant and free radical–scavenging properties. This system minimizes the impact of stress from single-cell passaging, helping to enable maximum cell viability and providing more efficient recovery of cells while retaining normal morphology, pluripotency, and karyotype.

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Definitive endoderm cells in 48 hours

The Gibco® PSC Definitive Endoderm Induction Kit is a fast way to generate high-quality, highly pure definitive endoderm cells from human pluripotent stem cells. The kit consists of two animal origin–free media, each supplied as a 1X complete medium, requiring no mixing of additional components. Unlike other methods that require multiple components and can take 5 days or more, the PSC Definitive Endoderm Induction Kit can generate ≥90% CXCR4+/PDGFRα– definitive endoderm cells that express the key markers Sox17 and FoxA2. The functional cells created from this kit are capable of differentiating to midgut/hindgut, pancreatic endoderm, or liver bud progenitors that express relevant physiological markers.

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Maximize PSC cryopreservation and recovery

The PSC Cryopreservation Kit contains a xeno-free cryopreservation medium and a chemically defined recovery supplement. When used in combination, these reagents help minimize loss of cell viability, maximize post-thaw recovery, and minimize unwanted differentiation of pluripotent stem cells (PSCs). The PSC Cryopreservation Kit is designed to consistently recover 50% more viable cells post-thaw compared to traditional methods of cryopreservation.

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Technical highlights

PSC Cardiomyocyte Differentiation Kit – More Efficient Path to Contracting Cardiomyocytes

The Gibco PSC Cardiomyocyte Differentiation Kit enables efficient differentiation of human pluripotent stem cells (hPSCs) to contracting cardiomyocytes in as few as 8 days. A newly developed protocol achieves even better cardiomyocyte differentiation efficiency by singularizing cells prior to differentiation. This allows for better seeding and confluency estimates, which have been shown to be crucial in maximizing differentiation efficiency.

See full details on the user bulletin under ‘Manuals and Protocols’

Publication: Characterizing Pluripotent Stem Cells Using the TaqMan hPSC Scorecard Panel

We recently published a Methods in Molecular Biology paper that featured the TaqMan hPSC Scorecard Panel. The paper, “Characterizing Pluripotent Stem Cells Using the TaqMan hPSC Scorecard Panel”, demonstrates the utility of the TaqMan hPSC Scorecard Panel for rapid characterization of pluripotent stem cell lines. It has now been chosen by Global Medical Discovery as a key scientific article contributing to excellence in biomedical research and was highlighted in the February series online.

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For Research Use Only. Not for use in diagnostic procedures.