Stem Cell Intel Issue 6
Each quarter, Stem Cell Intel will provide you with the latest product news, upcoming industry events, and easy access to technical tools such as publications, protocols, FAQs, and more. To receive Stem Cell Intel, please click the subscribe button below.
The best time you’ll have in Stockholm!
Visit us at booth B06:32 or B06:33 during the ISSCR 2015 Annual Meeting in Stockholm, Sweden. Some highlights of your experience this year include:
- ‘Humanity in a Dish’ - a focus session hosted by Dr. Chad Cowan of Harvard University
- Insightful innovation showcases featuring Alexander Meissner, Harvard Stem Cell Institute and Andrew Lee, Stem Cell Theranostics
- A chance to add a Gibco™ Life of Cells minifigure to your collection by participating in booth activities*
- The much-anticipated annual antics of David Harris the magician
See complete details at lifetechnologies.com/isscr2015
Coming Soon! Essential 8 Flex Medium
Essential 8™ Flex Medium provides all of the benefits of Essential 8 Medium; reduced variability of components, reliable and robust cultures with a xeno-free, cGMP produced media; along with the added benefit of flexibility. With Essential 8 Flex Medium, your pluripotent stem cell maintenance schedule is no longer controlled by the need to be in the lab every day to change the culture medium.
With Essential 8 Flex Medium, PSCs need to be fed only on the day of splitting and the day after, if the cells are to be left without feeding for more than 48 hours (the weekend), double the feed volume on Friday.
This workflow has been carried out for over 30 passages with cultures maintaining pluripotency, trilineage potential, and normal karyotype. Essential 8 Flex Medium, based upon the published formulation of Essential 8 Medium by Chen et al., allows for weekend-free pluripotent stem cell culture without a negative impact to the health of the stem cells.
Enabling simplified directed differentiation of human PSCs
Have you ever wished the process of directed stem cell differentiation was less laborious and mechanical so that you can get to studying the differentiated cells you are trying to produce? We have developed a suite of media products that enable differentiation of hPSCs to highly functional neural, cardiac, or endodermal lineages while reducing guesswork and effort associated with current methods.
View our differentiation portfolio
CTS Immune Cell Serum Replacement (SR) for T cell culture
Induced pluripotent stem cells (iPSCs) can be differentiated into many different cell types including human T lymphocytes where they have shown promise in providing an unlimited source of antigen-specific T cells targeting cancer.1These iPSC-derived T cells and T cells isolated from peripheral blood mononuclear cells (PBMCs) are expanded in vitro and typically require human serum–supplemented media, which can lead to variability and supply concerns. We have developed Gibco™ CTS™ Immune Cell SR, which can replace human serum in immune cell culture including T cell culture. CTS Immune Cell SR has been shown to support equivalent expansion of functional CART-19 T cells compared to human serum–supplemented media as demonstrated in in vitro expansion assays. A leukemia model in immunodeficient mice was used to determine the efficacy of CD19-specific CAR T cells grown in medium supplemented with CTS Immune Cell SR. CAR T cells were able to control leukemia cells two weeks after infusion, while control T cell–treated mice succumbed to leukemia. CTS Immune Cell SR may be a suitable replacement for human serum in culture media when expanding immune cells including T cells isolated from PBMCs or iPSC-derived T cells.
1. Themali M et al. (2013) Generation of tumor-targeted human T lymphocytes from induced pluripotent stem cells for cancer therapy. Nat Biotechnol 31(10):928–933.
For In Vitro Diagnostic Use.
Introduce CTS Immune Cell SR into your T cell culture today
Reliable characterization methods are critical when deriving and culturing diverse pluripotent stem cell (PSC) lines under different conditions. Our new “Pluripotent stem cell characterization methods” application note includes:
- A topical overview of common PSC characterization practices
- A summary of advantages and limitations of characterization practices
- Commonly used markers for characterizing undifferentiated PSCs, early germ layer specification, and differentiated cells (such as neurons and cardiomyocytes)
Ten selected presentations from the 2nd annual 24 Hours of Stem Cells event have been made available for your viewing pleasure. The free, on-demand webinars include research and technology presentations as well as presentations from keynote speakers Alexander Meissner and Guokai Chen. Enrich your perspective on stem cell research with these unique resources.
* Participation is open only to ISSCR-registered attendees 18 years or older (excluding vendors) who hand in the Passport book at either booth B06:32 or booth B06:33 between 15:15, Wednesday 24 June and 16:00, Saturday 27 June 2015. Participants who submit a completed Passport book will receive a Life of Cells mini-figure (retail value approximately £3). Limit one mini-figure per person. By accepting a gift, you warrant that you are not prohibited by employment, contract, or law from accepting a gift from Life Technologies. Health-care professionals may not participate in the promotion. Health-care professionals include clinical or non-clinical physicians (including MD and MDs/PhDs), and physician assistants, nurses, technicians, research coordinators, administrators and purchasing personnel as well as entities (including hospitals, rehabilitation centres, nursing facilities, home health agencies, clinics and group purchasing organizations, managed care organizations, physician group practices, medical directors for health insurance organizations, etc.) and their employees and representatives who are involved in the actual provision of health-care service or on the context of their profession, purchase, use and/or sell medical devices and medical products. Void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulations or agency/institutional policy.
For Research Use Only. Not for use in diagnostic procedures.