TaqMan® hPSC Scorecard Assay | Thermo Fisher Scientific

decorative with text “assess differentiation potential”

Use the TaqMan® hPSC Scorecard Panel when you need a fast, standardized way to verify pluripotent stem cell (PSC) identity and detect early lineage tendencies before committing to downstream differentiation. The panel provides 94 pre-plated assays with integrated cloud-based analysis, making it a practical tool for labs that want consistent benchmarking across experiments, collaborators, or sites.

On this page:

What is hPSC Scorecard Panel assay?
Why we chose the TaqMan® hPSC Scorecard assay
Order TaqMan® hPSC Scorecard Panel

How to run the hPSC Scorecard assay
How to prepare the TaqMan® hPSC Scorecard Panel

Quantitative analysis of trilineage differentiation potential of your PSCs

The Applied Biosystems TaqMan® hPSC Scorecard Panel assesses pluripotency and trilineage differentiation potential using real-time qPCR assays and intuitive data analysis software. The hPSC Scorecard assay was developed in collaboration with Alex Meissner, and follows from his landmark publication [1]. The accompanying analysis software gives researchers anywhere in the world the ability to compare the gene expression profiles of their cell lines to a common reference set.

What is hPSC Scorecard Panel Assay?

The TaqMan® hPSC Scorecard Panels is a quantitative assay used to confirm the pluripotency and trilineage differentiation potential for both human embryonic stem cells (hESCs) and induced pluripotent stem cell lines (iPSCs). The panel of genes was developed in collaboration with Alex Meissner and based on his publication [1].

Designed for fast standardized analysis, the panel includes

94 predefined TaqMan® Gene Expression assays (including endogenous controls) dried-down in assay plate wells
Available in 96- and 384-well formats for different throughput needs

Key benefits of TaqMan® hPSC Scorecard Panels

A quantitative assay for confirming pluripotency and trilineage differentiation potential
Comparison of expression profiles to a reference standard
An easy-to-use platform with pre-plated assays and dedicated, intuitive analysis software

How it works (protocol overview)

Test undifferentiated, spontaneous differentiation (EB) or directed (monolayer) differentiation samples
Extract and purify RNA
Generate cDNA
Run gene expression panel on a compatible qRT-PCR instrument
Upload .eds files to the hPSC Scorecard Analysis Software
Analyze data and export results from the convenient cloud-based analysis application

View protocol

Why we chose the TaqMan® hPSC Scorecard Assay

Hear from Jennifer Moore at RUCDR/Infinite Biologics on the benefits Scorecard has to offer.

Instrument requirements

See which instruments are compatible with the TaqMan® hPSC Scorecard Panel and how to set them up.

Data analysis

The TaqMan® hPSC Scorecard Panel includes access to free, intuitive web-based analysis software that allows researchers to quantitatively compare the gene expression profile of their sample to that of a reference set.