TaqMan TAMRA Probes

Custom Applied Biosystems™ TaqMan® TAMRA™ qPCR probes feature a 5’ reporter (Applied Biosystems™ FAM™, VIC™, or TET™ dye) and 3’ fluorescent quencher (TAMRA™ dye). TaqMan TAMRA probes, which were some of the first TaqMan probes to be developed, can be used for a variety of applications.

TAMRA is a fluorescent dye and therefore can be multiplexed with up to 3 dyes. (QSY probes are recommended for anything higher than triplex.) TAMRA probes are typically longer (30 to >40 bp) than MGB nonfluorescent quencher (NFQ) probes, and this can result in reduced specificity.

All TaqMan TAMRA probes are HPLC-purified and verified by mass spectrometry to help ensure lot-to-lot reproducibility between batches. Thermo Fisher Scientific has been a leader in qPCR probe manufacturing for over 20  years. TaqMan probes are manufactured in a facility that is certified to ISO 13485.