Premier HPLC & UHPLC Columns

We've got a column for that!

Solve your separation challenge with your new favorite column. Bringing you Thermo Scientific Accucore, Acclaim, and Hypersil GOLD premier HPLC and UHPLC columns, which feature:

  • High resolution, high pressure UHPLC and HPLC columns
  • A 40-year legacy of innovation
  • Unmatched variety of chemistries, particle sizes, and column formats
  • From the most popular C18 phases, to innovative mixed mode chemistries, we have the right column for your separation needs
  • Long lifetime and reproducible
  • The broadest range of solid core columns on the market

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Accucore

Solid core for high resolution

 

 

 

  • Fast separation on limited sample volume
  • Increased sensitivity using standard HPLC
  • High resolution without elevated backpressure

Acclaim

Fully porous for complicated samples

 

 

 

  • Complex mixtures
  • Higher resolution
  • More phases, more choices
     

Hypersil GOLD

Fully porous for simple samples

 

 

 

  • Great starter columns
  • Fast method development
  • Great for general analyses
     

Webinar

Venn diagram of overlaps between column types

Understanding Diversity in HPLC Chromatography Chemistries

Learn how to choose your HPLC chemistry

  • What’s the best column to maximize your productivity?
  • Understanding the wide spectrum of selectivities
  • Superficially porous vs. fully porous packing material
  • Unique phases for challenging sample mixtures

Watch webinar


Take a closer look

Accucore HPLC & UHPLC Columns

Discover more

  • High resolution separation on rugged solid core (superficially porous) particles
  • Fast and easy analyses with a wide selection of chemistries
  • Increased sensitivity on a standard HPLC system
  • Compatible with almost any instrument
  • An excellent return on investment

15 Accucore chemistries to choose from

Diagram of 15 Accucore chemistries to choose from in 5 categories: Polar selectivity stability in 100% aqueous conditions, C18 selectivity, Alternate hydrophobic selectivity, HILIC selectivity polar compound retention, and Aromatic selectivity orthogonal to C18

Accucore phase overview

Accucore Vanquish UHPLC columns

Using 1.5 μm solid core particles, allow UHPLC users to enjoy performance benefits over columns packed with conventional sub-2 μm fully porous particles

Phase name Particle type (μm) Carbon load (%) USP
C18+ 1.5 6.5 L1

Accucore HPLC columns for biomolecules

The range of Accucore HPLC columns packed with 150 Å pore diameter particles allows biomolecule separations to benefit from the superb resolution and high speed enabled by solid core technology.

Phase name Particle type (μm) Carbon load (%) USP
150-C18 2.6 7 L1
150-C4 2.6 2 L26
150-Amide-HILIC 2.6

Accucore XL HPLC columns

Using 4 μm solid core particles, Accucore XL HPLC columns allow users of conventional HPLC methods to enjoy performance far beyond that of columns packed with 5 μm, 4 μm or even 3 μm fully porous particles.

Phase name Particle type (μm) Carbon load (%) USP
C18 4.0 7 L1
C8 4.0 4 L7

Accucore HPLC columns

Containing solid core particles having a 2.6 μm diameter and a very narrow particle size distribution high speed, high resolution separations

Phase name Particle type (μm) Carbon load (%) USP
RP-MS 2.6 7 L1
C18 2.6 9 L1
C8 2.6 9 L7
aQ 2.6 9 L1
Polar Premium 2.6 8 L60
Biphenyl 2.6 6 L11
Phenyl-Hexyl 2.6 5 L11
PFP 2.6 5 L43
Phenyl-X 2.6 6
C30 2.6 5 L62
HILIC 2.6 L3
Urea-HILIC 2.6

Learn more about Accucore columns

Acclaim HPLC & UHPLC Columns

Resolve more

  • The go-to column for complex mixtures that require more resolution
  • More phases, more choices
  • Fully porous with a high surface area, ensuring high retention
  • Designed for complicated samples
  • Separates and resolves compounds not possible with other columns

21 Acclaim chemistries to choose from

General-purpose RP

Separate complex samples with high surface area columns (C18, etc).
 
 

Mixed-mode

Retain multiple types of analytes on a single column.
 
 

Application-specific

Unique columns for specific applications: surfactants, organic acids, pesticides, aminoglycosides, explosive residues.
 
 

HILIC

Designed for the separation of polar compounds.

21 Acclaim chemistries to choose from in 4 categories: General-purpose RP, Application-specific, HILIC, Mixed-mode

Acclaim phase overview

Phase Name 2.2 µm 3.0 µm 5.0 µm Pore Diameter (Å) Surface Area(m²/g) Carbon Load (%) USP
Acclaim 120 C18 yes yes yes 120 300 18 L1
Acclaim 120 C18 Vanquish yes 120 300 18 L1
Acclaim 120 C8 yes yes yes 120 300 11 L7
Acclaim 300 C18 yes 300 100 8 L1
Acclaim Polar Advantage yes yes yes 120 300 17 L60
Acclaim Polar Advantage 2 yes yes yes 120 300 17 L60
Acclaim PA2 Vanquish yes 120 300 17 L60
Acclaim C30 yes yes 200 200 13 L62
Acclaim Phenyl-1 yes yes 120 300 13 L11
Acclaim HILIC-10 yes 120 8
Acclaim Organic Acid yes yes 120 300 17
Acclaim Surfactant yes yes 120 300 12
Acclaim Surfactant Plus (for LC-MS / CAD) yes yes 120 300
Acclaim Explosives E2 yes yes yes 120 300
Acclaim Carbamate yes yes yes 120 300
Acclaim Carbonyl yes yes yes 120 300
Acclaim Trinity Q1 yes 300 100
Acclaim AmG C18 yes 120 300
Acclaim Trinity P1 yes 300 100
Acclaim Trinity P2 yes 300 100
Acclaim Mixed-Mode WAX-1 yes yes 120 300 L85
Acclaim Mixed-Mode HILIC-1 yes yes 120 300
Acclaim Mixed-Mode WCX-1 yes yes 120 300 L78
Acclaim SEC-1000 7µm 1,000 L38
Acclaim SEC-300 5µm 300 L25

Learn more about Acclaim columns

Hypersil GOLD HPLC & UHPLC Columns

Back to basics

  • The go-to, easy-to-use HPLC columns for many chromatographic separations
  • Built on 40 years of experience in column development and manufacturing
  • Outstanding peak shape for reversed phase, ion exchange, HILIC, or normal phase chromatography
  • Excellent resolution, efficiency and sensitivity

Complete range of Hypersil GOLD columns to start your chromatographic separation

Hypersil GOLD
Hypersil GOLD C8
Hypersil GOLD C4
Traditional reversed-phase columns for hydrophobic interaction; C8 and C4 are better when less hydrophobicity is needed.
Hypersil GOLD aQ Good for basic compound separations by reversed-phase using aqueous mobile phase.
Hypersil Phenyl
Hypersil PFP
Aromatic compounds are separated better with these columns.
Hypersil AX
Hypersil SAX
Hypersil Amino
Ion exchange columns are preferred for ionic compounds.
Hypersil HILIC
Hypersil Silica
Polar compounds are retained and separated well with these hydrophilic columns.
Complete range of Hypersil GOLD columns to start your chromatographic separation in 4 categories: Reversed-phase selectivity, Ion-exchange selectivity, HILIC selectivity, Aromatic selectivity

Hypersil GOLD phase overview

Phase Name 1.9 µm 3.0 µm 5.0 µm 12.0 µm Carbon Load (%) USP pH Range
Hypersil GOLD C18 yes yes yes yes 10 L1 2 to 11
Hypersil GOLD C8 yes yes yes 8 L7 2 to 9
Hypersil GOLD C4 yes yes yes 5 L26 2 to 8
Hypersil GOLD aQ yes yes yes 12 L1 2 to 9
Hypersil GOLD PFP yes yes yes 8 L43 2 to 8
Hypersil GOLD Phenyl yes yes yes 8 L11 2 to 8
Hypersil GOLD CN yes yes yes 4 L10 2 to 8
Hypersil GOLD Amino yes yes yes 2 L9 2 to 8
Hypersil GOLD AX yes yes yes 6 2 to 8
Hypersil GOLD SAX yes yes yes 2.5 L14 2 to 8
Hypersil GOLD Silica yes yes yes L3 2 to 8
Hypersil GOLD HILIC yes yes yes 6 2 to 8

Learn more about Hypersil GOLD columns



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