Go beyond what you thought possible with native MS

High-resolution native MS analysis

The Q Exactive UHMR Hybrid Quadrupole-Orbitrap Mass Spectrometer is Thermo Fisher Scientific's first platform to combine substantially increased sensitivity and mass resolution at high m/z, with MS2 and pseudo-MS3 capabilities – creating a unique and powerful analytical solution for the investigation of structure and heterogeneity of protein complexes. With its superior ability to perform native intact mass and top-down analyses, Q Exactive UHMR MS provides unprecedented structural detail which you cannot see with other methods enabling deeper understanding of protein function, disease mechanisms, potential drug targets, and biotherapeutic compounds.

Q Exactive UHMR and Vanquish UHPLC systems

Key benefits
Confidently resolve small mass differences Conserve precious sample Perform native top-down analysis of protein complexes Characterize intact native protein assemblies, including membrane proteins
Analyze intact MegaDalton assemblies and resolve small differences in masses that reveal key ligands, modifications and interactions for better understanding of biological processes. With orders of magnitude enhanced sensitivity at high m/z, make high-confidence measurements, even if you have very small amounts of sample to work with. Ultra-high mass quadrupole selection up to 25k m/z and higher fragmentation efficiency in the injection flatapole and HCD cell region allow native top-down analysis. By varying the in-source trapping energy, the instrument can release protein subunits for top-down sequencing or, with gentle activation, retain membrane proteins bound to multiple ligands allowing whole complex analysis.

How does in source trapping work?

In-source trapping gently desolvates non-covalent protein complexes and efficiently removes detergent micelles for the analysis of intact membrane proteins and protein complexes. When desired, in-source trapping provides dissociation of non-covalent protein complexes into subunits for subsequent top-down analysis.


Protein-nucleic acid assemblies

The Q Exactive UHMR mass spectrometer enables native MS analysis of large protein–nucleic acid assemblies that require high resolution and very efficient transmission in the ultrahigh mass range.

Intact membrane proteins

Novel in-source trapping permits native MS and native top-down analysis of membrane proteins and membrane protein complexes together in one instrument.

Protein-nucleic acid assemblies

The Q Exactive UHMR mass spectrometer enables native MS analysis of large protein–nucleic acid assemblies that require high resolution and very efficient transmission in the ultrahigh mass range.

Intact membrane proteins

Novel in-source trapping permits native MS and native top-down analysis of membrane proteins and membrane protein complexes together in one instrument.

Featured content

Video

Prof. Albert Heck, University of Utrecht, and Alexander Makarov, Thermo Fisher Scientific, discuss the developing field of native MS.

Video

Prof. Alexander Makarov takes us through the evolution of Obitrap technology for the analysis of biotherapeutics.

Case study

Oxford Mass Technologies (OMass) specializes in native mass spectrometry platforms for multidimensional analysis of challenging protein targets for the biopharmaceutical industry. Read about how they are using new technology for advanced protein characterization.


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