Cell imaging must go on.

Stay focused on your critical work to achieve clear and accurate imaging results.  Our breadth of imaging products from plastics and media to probes and dyes are optimized for quality to help control the experiment from start to finish and deliver reliable results.

New Alexa Fluor conjugated Nano Secondary Antibodies

Microscopic view of fluorescently stained cells in three colors

Invitrogen Alexa Fluor Nano secondary antibodies are a new and exciting class of fluorescently conjugated antibodies. These ‘nanobodies’, as they are often called, offer functional and time saving benefits, because of their unique structures that are distinct and much smaller than traditional antibodies. Nano secondary antibodies can be readily co-incubated with primary antibodies for fluorescence imaging and high-resolution microscopy applications. Basic and translational researchers can improve scientific productivity, prevent cross-reactivity, and pinpoint the specific primary target in a diverse set of samples and cell types with Invitrogen Alexa Fluor conjugated Nano secondary antibodies.

Learn more about New Alexa Fluor conjugated Nano Secondary Antibodies

Cell health and function assays

Microscopic image of fluorescently stained mammalian cells showing subcellular structures

Cell health and function assays

Understanding cellular function through imaging is a valuable tool for biology research. Thermo Fisher Scientific offers a broad range of cell function assays and reagents to elucidate key cellular processes including apoptosis, cell proliferation, viability and cytotoxicity, oxidative stress, and internalization processes like phagocytosis and endocytosis, as well as indicators for ion homeostasis.

Secondary antibodies

Microscopic image of fluorescently stained cells in three colors

Fluorescent secondary antibodies

Fluorescent conjugated secondary antibodies are a valuable tool for identifying cellular proteins in fluorescent imaging, western blotting, immunohistochemistry, and more. Alexa Fluor and Alexa Fluor Plus secondary antibodies offer advantages of brighter signal, multiplexing capabilities, and ease of use. Secondary antibodies are great for fixed cell imaging applications for subcellular protein targets that need a primary antibody to be identified.

Microscopic image of fluorescently stained tissue section

Enzyme and biotin labeled secondary antibodies

Invitrogen secondary antibodies offer both high-activity horseradish peroxidase and alkaline phosphatase secondary antibody conjugates for immunohistochemistry, western blotting, ELISA, or other enzyme-driven applications. Also available are a wide variety of biotin and DSB biotin conjugates that allow for use of the same secondary antibody in multiple applications, simply by switching the streptavidin being used.

Instrument calibration

Microscopic image of stained cell showing three fluorescent colors

Microscope calibration reagents

Regular instrument calibration and adjustment are required for high-precision imaging of fluorescent probes, particularly in multicolor applications such as confocal laser scanning microscopy that involve multiple exposures, repetitive scans, or three-dimensional sectioning. Invitrogen offers imaging dye standards, InSpeck intensity calibration beads, FocalCheck calibration beads and test slides, TetraSpeck microspheres, and FluoCells prepared slides.

Imaging dishes, plates, and coverglass

Cell culture dishes sitting on a microscope stage

Cell imaging plastics

Maximize performance with Thermo Scientific Nunc Cell Culture Imaging Products. We offer a variety of plastics for cell imaging applications in fluorescence microscopy, phase contrast microscopy, confocal microscopy, live cell imaging, and fluorescence in situ hybridization (FISH).

Imaging media, mountants, and antifades

Microscopic image of adherent cells in culture showing subcellular structures

Mounting media

ProLong and SlowFade mounting medias are exceptional at maintaining bright fluorescent signals and reducing photobleaching for imaging cells or tissue. By using high quality mountant and antifade medias, coupled with fluorescent imaging dyes or antibodies, you can increase fluorophore photostability in both live-cell and fixed-cell samples for immediate imaging and for long-term storage.

Bottle of FluoroBrite Media

FluoroBrite Media (Media for live cell imaging)

Gibco FluoroBrite DMEM media is formulated so that background fluorescence is 90% lower than that emitted by standard phenol red–free DMEM media. It is designed to enhance the signal-to-noise ratio of fluorophores so that researchers can visualize weaker fluorescent signals due to hard-to-detect or low-expressing targets–all while maintaining cellular health and preventing photobleaching.

Bottle of Balanced salt solutions to maintain the integrity of cells

Balanced salts–Washing cells (PBS)

Balanced salt solutions can provide an environment that maintains the structural and physiological integrity of cells, critical for cell imaging workflows, and for achieving the highest quality and consistent results.

ReadyProbes Ready-to-Use Imaging Reagents

Vials of ready-to-use reagents

ReadyProbes Ready-to-Use Imaging Reagents

Invitrogen ReadyProbes reagents are ready-to-use imaging solutions designed to allow you to stain cells with a simple protocol that does not require any calculation, dilution, or pipetting. ReadyProbes dropper bottles have been formulated with some of the most popular imaging reagents including fluorescent secondary antibodies, cellular organelle labels, and cell health probes.

Organelle counterstains