Big insights can come from small amounts of sample. The Invitrogen Collibri DNA library prep kits for Illumina systems are designed to improve sensitivity of variant detection from 1 ng of DNA compared to options from Illumina, Kapa Biosystems, or NEB. 1 ng of DNA is sufficient to determine copy number variation (CNV) from liquid biopsy research samples, assemble entire genomes, or even investigate gene regulation by ChIP-Seq. Collibri DNA library prep kits are suitable for genomes of all sizes and complexity.
The Collibri PS DNA Library Prep Kit for Illumina Systems efficiently converts cfDNA into libraries for sequencing on Illumina systems, with a few modifications to the standard protocol.
GC bias is the biggest contributor to uneven coverage, but it can be minimized by using an optimal PCR master mix or PCR-free library preparation methods.
Two common methods are used to fragment DNA: physical shearing and enzymatic shearing. The choice of method depends on factors such as amount of nucleic acid available and access to shearing equipment.
A comparison of qPCR and Qubit fluorometry for quantification of NGS libraries intended for sequencing on an Illumina NGS system.
Do you plan to analyze viral genomes on a MiSeq™ system? Or perhaps your project involves analysis of hundreds of human samples on a NovaSeq™ 6000 system? The Collibri DNA library prep kits generate sequencing-ready libraries that are compatible with all Illumina NGS systems.
For Research Use Only. Not for use in diagnostic procedures.
Illumina, NovaSeq, and MiSeq are trademarks of Illumina Inc. KAPA is a trademark of Roche Inc. NEB is a trademark of New England BioLabs.