Whole-Genome Library Prep Success

Compatible with all Illumina NGS systems

In 1.5 hours, generate NGS libraries from 1 ng of cell-free, FFPE, or genomic DNA
with consistent results every time

1 ng of DNA unlocks insights—all genomes welcome

Big insights can come from small amounts of sample. The Invitrogen Collibri DNA library prep kits for Illumina systems are designed to improve sensitivity of variant detection from 1 ng of DNA compared to options from Illumina, Kapa Biosystems, or NEB. 1 ng of DNA is sufficient to determine copy number variation (CNV) from liquid biopsy research samples, assemble entire genomes, or even investigate gene regulation by ChIP-Seq. Collibri DNA library prep kits are suitable for genomes of all sizes and complexity.

Application note

Library preparation from circulating cell-free DNA

The Collibri PS DNA Library Prep Kit for Illumina Systems efficiently converts cfDNA into libraries for sequencing on Illumina systems, with a few modifications to the standard protocol.

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minimize bias for sequencing on illuminating systems

Technical note

Scaling library preparation and maintaining quality

GC bias is the biggest contributor to uneven coverage, but it can be minimized by using an optimal PCR master mix or PCR-free library preparation methods.

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DNA fragmentation options for sequencing on illuminating systems

Technical note

DNA fragmentation strategies for NGS library preparation

Two common methods are used to fragment DNA: physical shearing and enzymatic shearing. The choice of method depends on factors such as amount of nucleic acid available and access to shearing equipment.

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library quantification strategies for sequencing on illumina systems

Technical note

Understanding library quantification assays for NGS applications

A comparison of qPCR and Qubit fluorometry for quantification of NGS libraries intended for sequencing on an Illumina NGS system.

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Compatible with all Illumina NGS systems

Do you plan to analyze viral genomes on a MiSeq™ system? Or perhaps your project involves analysis of hundreds of human samples on a NovaSeq™ 6000 system? The Collibri DNA library prep kits generate sequencing-ready libraries that are compatible with all Illumina NGS systems.