LAMP Applications for viral pathogen detection

Advance your viral testing and research with Loop-mediated isothermal amplification (LAMP) technology for simple, fast and affordable solutions for viral pathogen research and surveillance. Choose from our RT-LAMP products below to simplify and accelerate detection of RNA targets to optimize your research workflow.


LAMP technology is increasingly used in molecular diagnostics to accelerate the detection of viral, bacterial, and parasitic pathogens.
LAMP technology overview

LAMP Technology

Loop-mediated isothermal amplification (LAMP) is a simple, sensitive, fast and affordable solution for RNA and DNA detection, with several methods for evaluating results. With a simple workflow and fast reaction speed, it is useful for in field applications for faster detection and surveillance of viral pathogens.

LAMP used in SARS-CoV-2 research

SARS-CoV-2: Research and surveillance

Invitrogen Colorimetric ReadiLAMP™ SARS-CoV-2 Kit – assay for SARS-CoV-2 surveillance with simple workflow, and rapid results resulting in robust performance.

Invitrogen SuperScript IV RT-LAMP Master Mix – reagents for faster and simpler detection of RNA targets with maximum flexibility to optimize viral pathogen research and assay development for SARS-CoV-2, measles, influenza and other pathogens.

RT-LAMP Protocol and Application Note – standalone reagents for fast and simple testing and surveillance of viral pathogens, including SARS-CoV-2.

Additional RT-LAMP Applications

Other infectious diseases and applications

RT-LAMP reagents provide faster and simpler solutions to validate additional research and assay development.

Invitrogen SuperScript IV RT-LAMP Master Mix, and RT-LAMP Protocol and Application Note

  • Detection of pathogens (viruses, bacteria, fungi)
  • Molecular diagnostics research for food and water safety
  • Cancer research and diagnostics

RT-LAMP reagents, master mix and assay for research and surveillance of viral pathogens

RT-LAMP or reverse transcription loop-mediated isothermal amplification can simplify and accelerate your workflow to enhance detection of infectious diseases.

SARS-CoV-2
Other pathogens

The RT-LAMP protocol and application note includes standalone reagents for fast and simple testing and surveillance of viral pathogens, including SARS-CoV-2. It serves as a template for testing other targets using relevant primer sets.

SARS-CoV-2
Other pathogens

RT-LAMP master mix reagents for faster and simpler detection of RNA targets with maximum flexibility to optimize viral pathogen research for SARS-CoV-2, measles and influenza assay development.

Sample request

SARS-CoV-2

This innovative RUO kit utilizes non-invasive saliva or swab samples, delivers rapid results without use of any sophisticated equipment or personnel training, and can be used for frequent surveillance of SARS-CoV-2.



Resources

 Standalone enzymesInvitrogen SuperScript IV
RT-LAMP Master Mix
Invitrogen Colorimetric ReadiLAMP™ Kit, SARS-CoV-2
FormatCatalog reagentsMaster mixAssay kit
DescriptionProtocol and Application noteOptimized enzymatic reaction master mixOptimized colorimetric detection assay for SARS-CoV-2
ApplicationResearch & SurveillanceResearch & SurveillanceResearch & Surveillance
Optimal reaction temperature60°C65°C65°C
Reaction time30-50 min10-30 min30-60 min
(Direct LAMP option available)
PolymeraseBsm DNA PolymeraseProprietary Bst mutantProprietary Bst mutant
Reverse transcriptaseSuperScript IVSuperScript IVSuperScript IV
Additional additivesYesYesNo
DyeSYBR Green I and SYTO 9 / fluorescenceSYTO 9 / fluorescenceColorimetric indicator
SamplePurified RNA/DNAPurified RNA/DNAPurified RNA or Direct
SARS-CoV-2 specific primers and controls includedNoNoYes
Other than SARS targetsYesYesNo
Sophisticated instrumentsOptional
(Heat block or thermal cycler)
Optional
(Heat block or thermal cycler)
No
(Heat block)
Style Sheet for Global Design System