Product Manager, Protein and Cell Analysis
Thermo Fisher Scientific
Chemiluminescent detection is a widely-used method for detecting target proteins in a western blotting. This detection method is the result of an enzyme reaction of horseradish peroxidase (HRP) or alkaline phosphatase (AP) which produces light and can be measured by use of x-ray film or a CCD imager. Chemiluminescent substrates allow for various advantages over other methods in detection including, but not limited to, increased sensitivity, high signal-to noise ratios (or low background), and the ability to strip and reprobe the blot. Contrary to popular belief, however, "one size" does not fit all" when it comes to western blotting substrates. With so many options on the market, it is sometimes difficult to determine which substrate performs the best for individual western blot systems. In this webinar we will discuss the best methods for optimizing this technique as well as how to determine the appropriate reagents for your sensitive target detection.