M-MLV Reverse Transcriptase (200 U/µL)
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
Features of this enzyme:
- Thermostability – optimal activity at 37°C
- Size of cDNA – M-MLV can be used to synthesize first-strand cDNA up to 7 kb
- Applications – synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
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For Research Use Only. Not for use in diagnostic procedures.
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