M-MLV Reverse Transcriptase (200 U/µL)

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.

Features of this enzyme:

  • Thermostability – optimal activity at 37°C
  • Size of cDNA – M-MLV can be used to synthesize first-strand cDNA up to 7 kb
  • Applications – synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR

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