Simple NGS workflow for aneuploidy analysis

Ion ReproSeq PGS Kits for the Ion GeneStudio S5 System enable rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for laboratories interested in reliably assessing preimplantation embryo biopsy samples for research.

  • A simple and flexible throughput next-generation sequencing (NGS) workflow for aneuploidy analysis of embryo biopsy samples
  • Rapid workflow for 16, 24, or 96 samples per run; 10–13 hours from cells to analysed data
  • Enhanced interpretation of results with adjustable analysis workflows for increased sensitivity to detect small segmental aneuploidy and low-level mosaicism calling

With just 6 pg of DNA from a single cell, or input from multiple cells, these kits help provide accurate detection of whole-chromosome and chromosome-arm events in as little as 9.6 hours.*



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Figure 1. Aneuploidy detection from a single cell using an Ion ReproSeq PGS Kit for the Ion GeneStudio S5 System and Ion Reporter Software v5.4. The top panel illustrates a deviation (blue) from baseline, indicating a 45 Mb duplication of chromosome 9 in a male sample. The bottom panel indicates a deletion (red) of 48 Mb on chromosome 13 in a female sample.

Watch this short video to see the simple and scalable workflow in action

Cost-effective workflow that scales to your throughput needs

The Ion ReproSeq PGS Kits, along with the Ion GeneStudio S5 and Ion Chef Systems, deliver scalability and flexibility for various levels of sample throughput. Choose the kit that fits your throughput needs from a range of three sequencing chips to process between 16 and 96 samples.

Rapid turnaround for time-sensitive samples

Leveraging the speed of semiconductor sequencing and advances in template preparation on the Ion Chef System, Ion ReproSeq PGS Kits for the Ion GeneStudio S5 System offer a flexible benchtop NGS workflow for rapid aneuploidy analysis (Figures 2 and 3).

Enhanced interpretation of results

Ion Reporter Software makes analysing and reporting aneuploidy results simple and fast with specific workflows for mosaicism detection and gender masking, plus improved data plotting for easier data interpretation.

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Figure 2. Rapid total turnaround time for complete aneuploidy analysis using the Ion ReproSeq PGS Kits for the Ion GeneStudio S5 System. The comparisons are for the three Ion ReproSeq PGS Kits using the Ion GeneStudio S5 Plus System with their respective Ion chips and throughput as shown.

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Figure 3. Rapid workflow using Ion ReproSeq PGS Kits from a single cell to aneuploidy detection. Ion ReproSeq PGS Kits for the Ion GeneStudio S5 System include all materials for library construction, template preparation, and sequencing for the Ion GeneStudio S5 System workflow.

Find out more about the Ion ReproSeq PGS Kit.

For Research Use Only. Not for use in diagnostic procedures.