Full-Length & Uncut Full-Length cDNA Library Services
Uncut Libraries and the Power of Gateway™ Technology
To further enhance the chances of identifying full-length inserts, we have combined our proprietary full-length library procedure with the Gateway recombination-based cloning technology. Using Gateway technology allows us to avoid restriction enzyme digestion of the cDNA during library construction. Stringent quality control ensures that libraries are highly enriched for full-length cDNA clones (Figure 1 and Table 1). Our full-length cDNA libraries routinely achieve:
- 65–85% full-length clones (Table 2)
- >1 x 107 clones
- Insert lengths up to 8 kb
Figure 1. Estimation of the percentage of full-length inserts in six different cDNA libraries produced using Thermo Fisher Scientific’s proprietary full-length cDNA library technologies.
Table 1. Sample data for full-length human brain library. N=20
|Protein tyrosine kinase||3.0||71|
|Helix loop helix||4.0||80|
Table 2. Comparison of uncut full-length (FL) and full-length (FL) cDNA libraries.
|Major Difference||Uncut FL Library||FL Library|
|Vector||PENTR222||PCMVSport6.1 or pcDNA3.1|
|Starting Material (mRNA)||2-6ug||15ug|
|Res. Enzyme (Not I) Cut||No||Yes|
|Cloning Strategy||BxP Recombination||Ligation|
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