Uncut, Micro-, and Nanoquantity/Uncut Library Services
Finding Full-Length Targets With Uncut cDNA Libraries
- >90% recombinants
- High percentage of full-length clones
- Very high cloning efficiency
Table 1. Sample data for uncut cDNA libraries.
|Fractionation method||Total clones sequenced||Insert range (kb)||Average insert size (kb)||Full-length ORF|
|gel at 3.5 kb and up||36||0.65-7.1||2.8||12 (33%)|
We routinely obtain 5–10 x 106 clones from 30 ng of fractionated cDNA. Maximize your chance of obtaining full-length target sequences by choosing our uncut library option.
Microquantity Libraries: Scarce Samples Are Not a Problem
The ability to make cDNA libraries from small amounts of RNA (as little as 100 µg) or tissue (as few as 100 mg) is the hallmark of our latest generation of cDNA technologies, microquantity cDNA synthesis. This technology, in conjunction with our new nanoquantity mRNA isolation system and patented SuperScript™ Reverse Transcriptase, allows for the construction of directionally cloned cDNA libraries from rare and small tissue samples, cultured cells, or RNA. The technology is not PCR-based.
Nanoquantity Libraries: Really Scarce Samples Are Not a Problem, Either
Table 2. Construction of nanoquantity cDNA libraries from different amounts of tissue/cell/mRNA.
|Amount of tissue/cell/mRNA||Average insert size (kb)||% insert||Titer (cfu/ml)||Total primary cfu|
|1 mg Tissue (Rat Brain)||1.3||100%||0.11 x 106||1.30 x 106|
|5 mg Tissue (Rat Brain)||1.8||100%||1.30 x 106||15.6 x 106|
|5 x 104 cells (293 Cell)||1.3||91%||0.18 x 106||2.16 x 106|
|5 x 105 cells (293 Cell)||1.6||100%||6.60 x 106||79.0 x 106|
|5 ng mRNA (HeLa Cell)||1.4||91%||0.04 x 106||0.48 x 106|
|10 ng mRNA (Invertebrate Embryo)||2.2||100%||0.03 x 106||0.36 x 106|
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