The SelectScreen Cell-based GPCR Profiling Service utilizes Thermo Fisher Scientific's growing portfolio of division arrested or cryo-preserved GeneBLAzer™ and Tango™ functionally validated assays for GPCRs.

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It offers a flexible approach to screening:

  • Obtain rapid profiling of your compounds against a panel of GPCRs at a single concentration, or
  • Have more in-depth EC50/IC­50 dose response determinations performed.

You also chose whether you want your compounds profiled in agonist or antagonist mode. All of our functional cell lines are offered in both modes. Counter-screen your “hits” against our parental cell lines when utilizing our GeneBLAzer platform to evaluate non-specific effects.

Overview of GeneBLAzer Technology

Overview of Tango Technology

SelectScreen GPCR Profiling Service Panel Selections

This service offers all of the following:

  • High quality data
  • 2-3 week turnaround
  • Competitive pricing
  • Complete confidentiality
  • Open business model – NO minimum commitment

SelectScreen Cell-based GPCR Profiling Service Overview

Built-in Quality

A stringent validation process ensures the highest quality data possible. Each data point is performed in duplicate. Strict quality control protocols ensure that any assay results not meeting set specifications will be automatically repeated. EC50 or IC50 value determinations for each GPCR are performed with known agonist and/or antagonist (when available) to determine data validity and consistency.

More detail around the protocol, specifications, and controls can be obtained in our customer protocol and assay conditions guide, which is available in the PDF downloads on the right.

All of the functional, GPCR-specific GeneBLAzer and Tango cell lines used in the SelectScreen® Cell-based GPCR Profiling (SSCG) Service provide superior response profiles as a result of optimization of assay conditions and selection of high-responding cell populations. All GeneBLAzer and Tango cell lines in the portfolio were researched and developed by Invitrogen; we ensure the consistency, reliability, and performance of each cell line. In fact, the same cell lines that we use in our service can be purchased by our clients to perform internal follow-up experiments. GPCR-specific GeneBLAzer and Tango™ cell lines:

  • Are division arrested or cryo-preserved to avoid variability associated with cell division
  • Provide ready-to-screen, ratiometric assays for disease relevant targets
  • Are functionally validated to ensure high-quality results each and every time

The GeneBLAzer and Tango cell lines in the Service are tested and documented to show a high level of performance. These assays meet the following specifications:

  1. Z´-factor of 0.5 or greater for agonist assays and Z´-factor of 0.4 or greater for antagonist assays
  2. Appropriate EC50/IC50 responses to known agonists and antagonists

Listed below are the GPCR targets available for profiling.  Our list of targets represents a cross section of GPCR families, with most of the GPCRs falling into the Class A category of GPCR targets, with a smaller number in Class B. We will continue to expand our panel of GPCR targets, with emphasis on a broad representation of as many families as possible.  Check back often for updates to this list.

If your target of interest is not on the list below, additional catalog GPCR cell lines may be profiled using our Custom Services.  View the entire list of GPCR cell lines available.

If you identify a GPCR target you are interested in screening, please contact us at discoveryservices@thermofisher.com

Family Receptor Parental Cell Line
Acetylcholine (Muscarinic) M1 NFAT-bla CHO-K1
Acetylcholine (Muscarinic) M2 U2OS
Acetylcholine (Muscarinic) M3 NFAT-bla CHO-K1
Acetylcholine (Muscarinic) M4 Gqo5-NFAT-bla CHO-K1
Acetylcholine (Muscarinic) M4 U2OS
Acetylcholine (Muscarinic) M5 NFAT-bla CHO-K1
Adenosine ADORA1 U2OS
Adenosine ADORA3
U2OS
Adrenoreceptor ADRA1A NFAT-bla CHO-K1
Adrenoreceptor ADRA1B NFAT-bla CHO-K1
Adrenoreceptor ADRA2A Gqo5-NFAT-bla CHO-K1
Adrenoreceptor ADRA2A U2OS
Adrenoreceptor ADRB1 CRE-bla CHO-K1
Adrenoreceptor ADRB3 CRE-bla CHO-K1
Angiotensin AGTR1 U2OS
Angiotensin-Like AGTRL1 U2OS
Bradykinin B1 NFAT-bla CHO-K1
Bradykinin B2
NFAT-bla CHO-K1
Calcitonin CALCR CRE-bla Freestyle 293F
Calcitonin CALCRL:RAMP1 CRE-bla Freestyle 293F
Calcitonin CALCRL:RAMP3 CRE-bla Freestyle 293F
Calcium Sensing CaSR Gqo5-NFAT-bla CHO-K1
Cannabinoid CNR1 Galpha15-NFAT-bla CHO-K1
Cannabinoid CNR2 U2OS
Chemokine CCR1 U2OS
Chemokine CCR2 U2OS
Chemokine CCR3 U2OS
Chemokine CCR4 U2OS
Chemokine CCR5 U2OS
Chemokine CCR6 U2OS
Chemokine CCR7 U2OS
Chemokine CXCR1 U2OS
Chemokine CXCR2 U2OS
Chemokine CXCR3 U2OS
Chemokine CXCR4 U2OS
Chemokine CXCR6 U2OS
Chemokine CXCR7 U2OS
Chemokine Like CMKLR1 U2OS
Cholecystokinin CCKAR NFAT-bla HEK 293T
Cholecystokinin CCKBR NFAT-bla HEK 293T
Complement Component C5AR1 Galpha15-NFAT-bla CHO-K1
Corticotropin Releasing Factor CRHR1 CRE-bla CHO-K1
Corticotropin Releasing Factor CRHR2 CRE-bla CHO-K1
Dopamine D1 CRE-bla CHO-K1
Dopamine D2 Gqo5-NFAT-bla CHO-K1
Endothelial Differentiation Gene EDG1 U2OS
Endothelial Differentiation Gene EDG2 U2OS
Endothelial Differentiation Gene EDG3 Galpha15-NFAT-bla HEK 293T
Endothelial Differentiation Gene EDG4 U2OS
Endothelial Differentiation Gene EDG6 U2OS
Endothelial Differentiation Gene EDG7 NFAT-bla HEK 293T
Endothelial Differentiation Gene EDG8 U2OS
Endothelin EDNRA NFAT-bla HEK 293T
Endothelin EDNRA U2OS
Endothelin EDNRB NFAT-bla HEK 293T
Formyl Peptide-Like FPRL1 Galpha15-NFAT-bla CHO-K1
Galanin GALR1 U2OS
Galanin GALR2 U2OS
Gastrin Releasgin Peptide GRPR NFAT-bla CHO-K1
Ghrelin
GHSR
U2OS
Glucagon GCGR
CRE-bla CHO-K1
Glucagon GIPR
CRE-bla HEK 293T
Glucagon GLP1R CRE-bla CHO-K1
Glucagon GLP2R
U2OS
Gonadotrophin-Releasing Hormone
GnRHR
NFAT-bla CHO-K1
Histamine H1 NFAT-bla HEK 293T
Histamine H2 CRE-bla HEK 293T
Histamine H3 U2OS
Histamine H4 U2OS
KiSS1 GPR54 NFAT-bla CHO-K1
Leukotriene cysLT2 NFAT-bla CHO-K1
Leukotriene LTB4R Galpha15-NFAT-bla CHO-K1
Melanin-Concentrating Hormone MCHR1 (GPR24) Gqi5-NFAT-bla CHO-K1
Melanin-Concentrating Hormone MCHR2 U2OS
Melanin-Concentrating Hormone MCHR2 NFAT-bla CHO-K1
Melanocortin MC1R CRE-bla CHO-K1
Melanocortin MC2R CRE-bla CHO-K1
Melanocortin MC3R CRE-bla CHO-K1
Melanocortin MC4R CRE-bla CHO-K1
Melanocortin MC5R CRE-bla CHO-K1
Melatonin MTNR1A U2OS
Melatonin MTNR1B U2OS
Neuromedin U NMUR1 NFAT-bla CHO-K1
Neuropeptide S NPSR1-A NFAT-bla CHO-K1
Neuropeptide S NPSR1-B NFAT-bla CHO-K1
Neuropeptide W GPR8 U2OS
Neuropeptide Y NPY1R U20S
Neuropeptide Y NPY2R U2OS
Neurotensin NTSR1 NFAT-bla CHO-K1
Nicotinic Acid GPR109A U2OS
Opioid OPRD1 U2OS
Opioid OPRK1 Gqo5-NFAT-bla CHO-K1
Opioid OPRK1 U2OS
Opioid OPRL1 Gqi5-NFAT-bla Freestyle 293F
Opioid OPRL1 U2OS
Opioid OPRM1 U2OS
Orexin HCRTR1 NFAT-bla CHO-K1
Orexin HCRTR2 Galpha15-NFAT-bla CHO-K1
Orphan
GPR1
U2OS
Oprhan GPR119 CRE-bla CHO-K1
Oprhan GPR35
U2OS
P2Y P2RY11 NFAT-bla CHO-K1
P2Y P2RY2 NFAT-bla CHO-K1
P2Y P2RY6 NFAT-bla CHO-K1
Platelet Activating Factor PTAFR U2OS
Prolactin Releasing Peptide GPR10 NFAT-bla CHO-K1
Prostanoid
GPR44 (CRTH2)
U2OS
Prostanoid PTGDR CRE-bla CHO-K1
Prostanoid PTGER1
NFAT-bla CHO-K1
Prostanoid PTGER2 CRE-bla CHO-K1
Prostanoid PTGIR
CRE-bla CHO-K1
Prostanoid TBXA2R U2OS
Protease Activated F2LR1 U2OS
Relaxin RLN3R1 U2OS
Secretin SCTR CRE-bla CHO-K1
Serotonin HTR1A Galpha15-NFAT-bla CHO-K1
Serotonin HTR1A U2OS
Serotonin HTR2A U2OS
Serotonin HTR2B
NFAT-bla CHO-K1
Serotonin HTR7 CRE-bla CHO-K1
Somatostatin SSTR1 U2OS
Somatostatin SSTR2 U2OS
Somatostatin SSTR5 U2OS
Tachykinin TACR1 U2OS
Tachykinin TACR2 NFAT-bla CHO-K1
Tachykinin TACR3 U2OS
Vasopressin & Oxytocin
AVPR1A NFAT-bla CHO-K1
Vasopressin & Oxytocin
AVPR2 CRE-bla CHO-K1
Vasopressin & Oxytocin OXTR
Gqo5-NFAT-bla CHO-K1
VIP & PACAP PAC1 CRE-bla CHO-K1
VIP & PACAP VPAC1 CRE-bla CHO-K1
VIP & PACAP VPAC2 CRE-bla CHO-K1

For Research Use Only. Not for use in diagnostic procedures.