DNA templates for translation using "coupled" or "linked" transcription:translation systems can be easily generated by PCR. Below are the upstream (5') primer sequences to produce PCR products for T7-driven transcription and subsequent translation in a retic lysate and E. coli extract, respectively. Note, translation systems that use T3 or SP6 polymerases are also available . To generate PCR templates for other polymerases, simply change the T7 Promoter Sequence to a T3 or an SP6 sequence.