Enabling accurate gene representation with high performing SuperScript® III Reverse Transcriptase (RT)

  • High cDNA yields - greater thermostability provides a long half-life of typically 220 min at 50°C
  • More full-length cDNA - 50°C reaction temperature helps overcome RNA secondary structure
  • Increased specificity with gene-specific primers - retains full activity at 50°C

Reagents & Kits for RT

Choose SuperScript® III RT for robust first-strand synthesis and great cDNA yields. Engineered to be RNase H minus and thermostable up to 55°C, SuperScript® III RT delivers best-in-class cDNA synthesis in every single reaction, combining maximum yield with maximum sensitivity. You get full-length cDNA without degrading rare RNA transcripts. SuperScript®III RT always delivers the results you need to move forward - the first time, every time.

High-yield one-step RT-PCR for cloning

Complete RT-PCR system - SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity

  • Superior product yields from targets up to 9 kb (Figure 1)
  • Sensitive detection from as little as 1 pg HeLa total RNA
  • Simple one-tube, one-step reaction format
  • Direct compatibility with multiple applications, including multiplex RT-PCR and cloning

The SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity provides high product yields and sequence fidelity for applications such as cloning, gene detection, and quantification. The enhanced thermostability of SuperScript® III RT enables superior cDNA yields, while the addition of Platinum® Taq DNA Polymerase High Fidelity helps deliver full-length, high-fidelity PCR.

Figure 1.
High fidelity and performance of the SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity. One-step RT-PCR reactions were performed according to each manufacturer’s recommendations using the primers and starting HeLa total RNA quantities indicated. The thermostability and reduced RNase H activity of SuperScript® III RT is important for tackling RNA with secondary structure. SuperScript® III RT provides higher yields of full-length cDNA compared to other reverse transcriptase enzymes.

Time Saving Supermix

The SuperScript® III First-Strand Synthesis SuperMix is designed to provide high yields of first strand cDNA in a convenient highthroughput supermix format. The simple, time saving reaction setup uses just two tubes - a 2X reaction mix and a SuperScript® III enzyme mix (Figure 2).

An optional annealing buffer is provided for improved yields and sensitivity. The Enzyme Mix includes Super-Script® III Reverse Transcriptase to enable higher cDNA yields, superior sensitivity down to 1 pg and specificity, and compatibility with a wide variety of RT-PCR applications.

Figure 2:
SuperScript® III First-Strand Synthesis SuperMix provides a convenient time-saving reaction setup.