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Basic cell culture techniques for consistent results

Gibco Cell Culture Basics is an introduction to cell culture, covering topics such as laboratory setup, safety, and aseptic technique. You'll also find basic cell culture techniques and methods for passaging, freezing, and thawing cultured cells. Start here if you are new to cell culture techniques or need to refresh your knowledge.

To accompany the tips and tricks found here, we’ve also developed a companion handbook that introduces the fundamentals of cell culture. Download a copy to keep as a frequent reference guide.

Download the handbook    Explore all topics

Gibco Cell Culture Basics videos

Watch the latest Gibco Cell Culture Basics videos to learn valuable cell culture techniques to fuel your success.

Complete the training course and earn a badge to certify your knowledge

screenshot of Cell Culture Virtual Lab

The virtual training lab is an introduction to cell culture techniques, covering many topics including:

• Basic principles of cell culture • Preparing a new cell culture
• Performing a cell passage • Cryopreservation
artistic rendition of cow, cells and fbs bottle

Fetal Bovine Serum (FBS) Basics

Discover the important factors to consider in FBS selection, the market scenarios that drive FBS availability and price, as well as the important terms you must know and use when searching for the right FBS for your research needs.

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Gibco Cell Culture Basics Handbook

One Cell Biology Product Handbook

One Cell Biology Product Handbook cover

We have simplified our portfolio to show you how our most popular products and services are used as single tools or integrated system solutions. This handbook is meant to expedite your search for the right cell biology product and give you an in-depth look at how we can help accelerate research.

Learn more

Coaching to optimize your techniques

Course description
Mammalian cell culture is an integral tool in many workflows of biological study, and this course is designed to provide key cell culture techniques and best practices for maintaining healthy cell lines and obtaining the most accurate and reproducible results in downstream applications.

Who should attend?
This basic-level course is designed for life science professionals or students with little or no direct experience working in a tissue culture laboratory, or those wishing to refresh or expand on past experience.

Prerequisites
You should have read the Gibco Cell Culture Basics Handbook and completed the Gibco Cell Culture Basics virtual lab.

Complete the training course and earn a certificate to certify your knowledge

Complete the training course and earn a badge to certify your knowledge

screenshot of Cell Culture Virtual Lab

The virtual training lab is an introduction to cell culture techniques, covering many topics including:

• Basic principles of cell culture • Preparing a new cell culture
• Performing a cell passage • Cryopreservation
artistic rendition of cow, cells and fbs bottle

Fetal Bovine Serum (FBS) Basics

Discover the important factors to consider in FBS selection, the market scenarios that drive FBS availability and price, as well as the important terms you must know and use when searching for the right FBS for your research needs.

Explore now

Gibco Cell Culture Basics Handbook

One Cell Biology Product Handbook

One Cell Biology Product Handbook cover

We have simplified our portfolio to show you how our most popular products and services are used as single tools or integrated system solutions. This handbook is meant to expedite your search for the right cell biology product and give you an in-depth look at how we can help accelerate research.

Learn more

Coaching to optimize your techniques

Course description
Mammalian cell culture is an integral tool in many workflows of biological study, and this course is designed to provide key cell culture techniques and best practices for maintaining healthy cell lines and obtaining the most accurate and reproducible results in downstream applications.

Who should attend?
This basic-level course is designed for life science professionals or students with little or no direct experience working in a tissue culture laboratory, or those wishing to refresh or expand on past experience.

Prerequisites
You should have read the Gibco Cell Culture Basics Handbook and completed the Gibco Cell Culture Basics virtual lab.

Complete the training course and earn a certificate to certify your knowledge

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Endorsed by The American Association for Cell Biology

ASCB logo

The Gibco cell culture basics lab is endorsed by The American Association for Cell Biology (ASCB). Gibco and ASCB are committed to cell biology education. As part of our partnership you will receive discounted membership to ASCB after completing the virtual lab.

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We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.

Learn more about sustainable solutions

Resources

Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.

Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.

Related products

Support

Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

Need technical support? Contact our expert team for technical and application support of Laboratory Products