If you have asked, “What is fetal bovine serum and what is it used for?” you are not alone. Fetal bovine serum (FBS) is the liquid fraction remaining after the blood drawn from bovine fetus coagulates.1 Through centrifugation, cells, coagulation fibrinogens, and proteins are removed to produce serum.
Although fetal bovine serum has cells and clotting factors removed, over a thousand components conducive to cell growth remain, including:
Bovine serum is classified according to the age of the animal from which the blood was collected:2
The age of the animal is a noteworthy characteristic because it can impact the composition of the blood and, consequently, the serum.
Wondering what fetal bovine serum does and how it’s used in research? While other animal sera (e.g., horse, rabbit, goat, porcine, etc.) are available and utilized, fetal bovine serum remains the most universally employed. Fetal bovine serum contains a sparse amount of gamma globulin, higher levels of growth factors, and fewer complement proteins than both calf and adult bovine serum.
This makes FBS ideal for propagating cell growth while also decreasing the possibility of mammalian cells binding or lysing in the culture, rationalizing the preference of fetal bovine over newborn, donor, or adult bovine serum.
In which case, it’s likely you’re wondering “what is fetal bovine serum used for? What purpose does it serve?
In the late 1950s, Theodore Puck first introduced the use of FBS with the purpose of encouraging cellular proliferation. For several decades, the use of fetal bovine serum has been ubiquitous across laboratories worldwide. Its popularity has continued to lay in its indispensability as a supplement in cell and tissue culture applications.
Research, pharmaceutical, and biotechnical manufacturing have relied on FBS’s valuable properties. Its uses include but are not limited to:
Although fetal bovine serum collection has wide application, it is most frequently sought after for in vitro cell culture of eukaryotic cells.3 Supplementing culture media with animal serum primarily aids in:
The general procedure for fetal bovine serum collection includes the following phases:
FBS should always be stored at ≤-10°C until its intended use
While the process of fetal bovine blood collection is simple, the careful and controlled processing of FBS can be particularly extensive, depending on its intended useand required testing.
While filter pores can remove fungi and bacteria, they are unable to eradicate viruses. So, each batch is viral tested. Depending on customer requirements, the serum may be gamma irradiated to deactivate viral load.
Examples of testing conducted include:
Each stage of treatment is stringently documented for every batch, but customers are also able to test products prior to purchasing. Based on application, additional tests may be conducted.
In addition to gamma irradiation, there are several special treatments sera may undergo:
As a biological substance, FBS must be taken care of and appropriately stored to avoid degradation. The most effective manner of storage is by keeping the sera frozen.
FBS should be:
Government agencies (DEFRA, USDA, MPI, DAWR etc) set animal by-product standards and the requirements/regulations for the import and export of those products globally.
However, certain geographical regions have varying levels of viral risk. FBS imported from Canada and New Zealand into the US, for instance, require no further safety testing by the USDA. On the other hand, Australian sourced FBS must be tested for two viruses, Akabane and Bluetongue. Mexican and Central American FBS also require USDA safety testing for Bluetongue upon import into the US.
The International Serum Industry Association (ISIA) was established in 2006 to regulate the animal serum industry.4 ISIA created standards that require precise examination and verification of quality. The four key focus areas are:
The ISIA Traceability Program previously mentioned is not a required measure but a process that companies undergo willingly to certify serum traceability.5 ISIA approved and trained auditors will identify and report discrepancies from the first to the very last stage of FBS production. Review of records are required for certification.
Following the completion of the audit, qualified businesses may receive certification attesting to its fulfillment of standards. ISIA ultimately endorses that product labels and descriptions are accurate and authentic.
In addition to the ISIA Traceability program, fingerprinting technology has been innovated to push transparency in the sera collection and distribution process. Produced by Oritain, fetal bovine serum fingerprinting technology takes the measurements of natural elements absorbed by animals, plants, and soil, and then constructs a fingerprint.
There are three stages to the collection process:
This fingerprint allows verification of each serum’s origin. The origins of sera are crucial because bovine blood must only be sourced from countries with an approved animal health status, as well as from licensed facilities. Thus, via fingerprinting, consumers can be reassured that their products are genuine and from the geographic location as advertised.
Within recent years, the price of FBS has increased by over 300% in response to growing demand and restricted availability.6 Its supply is largely dependent on environmental factors and federal farming guidelines.
While there has been an uptick in contenders for FBS alternatives, there has yet to be conclusive results to demonstrate comparable efficacy.
However, the quality, safety, consistency, and regulatory compliance of Thermo Fisher Scientific products including sera are warranted. We ensure that our Gibco products undergo strict testing parameters, including ISIA Traceability Certified, and Fingerprinting Origin Guaranteed.
Remain informed about fetal bovine serum and rely on Thermo Fisher Scientific for a trustworthy source of FBS.
For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.