To concentrate cells from a suspension culture (or resuspended cells from monolayer culture):
Transfer the cell suspension to a sterile centrifuge tube of appropriate size and centrifuge for 10 minutes at 800 × g.
Note: Certain cell lines are very sensitive to centrifugal force.
- Carefully remove the supernatant without disturbing the cell pellet.
- Add the desired volume of fresh medium gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cell pellet.
- Transfer the cells to the desired, sterile container.