Cotransfection refers to the simultaneous transfection with two separate nucleic acid molecules, such as plasmid DNA and siRNA. Cotransfection is a common procedure for stable transfection. The plasmid DNA may contain a gene that is easily assayed and acts as a marker.
For this to occur, a marker gene is co-transfected, which gives the cell a selection advantage, for example resistance towards a certain toxin. Selecting the right reagent for cotransfection is essential.
Featured cotransfection reagents
Lipofectamine® 3000 reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types with improved cell viability.
- Learn more about Lipofectamine® 3000
- Learn more about Lipofectamine® 2000
Cotransfection Protocol with Lipofectamine® 2000
- View the Cotransfection protocol