The most complete fluorescent labeling and detection reference available, The Molecular Probes Handbook—A Guide to Fluorescent Probes and Labeling Technologies contains over 3,000 reagents and kits representing a wide range of Invitrogen™ Molecular Probes™ labeling and detection products. The significantly revised 11th edition features extensive references, reorganized content, and new technical notes and product highlights.

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Table of Contents

Fluorescence Fundamentals


Part I—Choosing a Fluorescent Label: Their Properties and Labeling Chemistries

Chapter 1 — Fluorophores and Their Amine-Reactive Derivatives
Chapter 2 — Thiol-Reactive Probes
Chapter 3 — Click Chemistry and Other Functional Group Modifications
Chapter 4 — Biotin and Hapten Derivatives
Chapter 5 — Crosslinking and Photoactivatable Reagents


Part II—Detecting Biomolecules: Signal Amplification and Secondary Detection

Chapter 6 — Ultrasensitive Detection Technology
Chapter 7 — Antibodies, Avidins and Lectins

Part III—Decoding Cell Components: Nucleic Acid and Protein Analysis

Chapter 8 — Nucleic Acid Detection and Analysis
Chapter 9 — Protein Detection and Analysis
Chapter 10 — Enzyme Substrates and Assays

Part IV—Probing Cell Structure: From Organelles and Membranes to Whole Cells

Chapter 11 — Probes for Cytoskeletal Proteins
Chapter 12 — Probes for Organelles
Chapter 13 — Probes for Lipids and Membranes
Chapter 14 — Fluorescent Tracers of Cell Morphology and Fluid Flow


Part V—Probing Cell Function: From Cell Viability to Ion Flux

Chapter 15 — Assays for Cell Viability, Proliferation and Function
Chapter 16 — Probes for Endocytosis, Receptors and Ion Channels
Chapter 17 — Probes for Signal Transduction
Chapter 18 — Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19 — Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20 — pH Indicators
Chapter 21 — Indicators for Na+, K+, Cl and Miscellaneous Ions
Chapter 22 — Probes for Membrane Potential


Part VI—Integrating Technology and Instrumentation

Chapter 23 — Antifades and Other Tools for Fluorescence Applications


Supplementary Information

Technical Notes and Product Highlights
List of Tables 

Resources

Research tools

 

Technical resources

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