Table 8.2 Cell membrane–impermeant cyanine nucleic acid stains.

Cat. No. Dye Ex/Em * MW †
SYTOX Dyes: Dead-Cell Stains ‡
S11348
S34857
SYTOX Blue 445/470 ~400
S7020
S34860
SYTOX Green 504/523 ~600
S11368
S34861
SYTOX Orange 547/570 ~500
S34859 SYTOX Red 640/658 ~450
Cyanine Dimers: High-Affinity Stains §  
P3580 POPO-1 434/456 1170.53
B3582 BOBO-1 462/481 1202.66
Y3601 YOYO-1 491/509 1270.65
T3600 TOTO-1 514/533 1302.78
J11372 JOJO-1 529/545 1272.63
P3584 POPO-3 534/570 1222.61
L11376 LOLO-1 565/579 1462.54
B3586 BOBO-3 570/602 1254.73
Y3606 YOYO-3 612/631 1322.73
T3604 TOTO-3 642/660 1354.85
N7565 Dimer Sampler Kit ** Various Various
Cyanine Monomers: Nuclear Counterstains ††
P3581 PO-PRO-1 435/455 579.26
Y3603 YO-PRO-1 491/509 629.32
T3602 TO-PRO-1 515/531 645.38
J11373 JO-PRO-1 530/546 630.31
P3585 PO-PRO-3 539/567 605.30
Y3607 YO-PRO-3 612/631 655.36
T3605 TO-PRO-3 642/661 671.42
T7596 TO-PRO-5 747/770 697.46
* Wavelengths of excitation (Ex) and emission (Em) maxima, in nm. All excitation and emission maxima were determined for dyes bound to double-stranded calf thymus DNA in aqueous solution. † Molecular weight (MW) for the anhydrous compound. The preceding ~ symbol indicates an approximate value, not including counterions. ‡ Products (S11348, S7020, S11368) supplied as 250 µL of a 5 mM solution; flow cytometry products (S34857, S34860, S34861) supplied as 1 mL of a 1 mM solution; SYTOX Red Dead Cell Stain (S34859) is supplied as 1 mL of a 5 µM solution. § Products (except N7565) supplied as 200 µL of a 1 mM solution. ** Includes 10 µL each of a 1 mM solution of the TOTO-1, TOTO-3, YOYO-1, YOYO-3, BOBO-1, BOBO-3, POPO-1 and POPO-3 dyes. †† Products supplied as 1 mL of a 1 mM solution.