Table 15.5 Fluorogenic substrates for caspase activity.
|Peptide Sequence||Target for Caspases *||
|DEVD||3, 6, 7, 8, 10||E13184 ‡
|IETD||8, granzyme B||R22125
|* Caspase substrates can often be cleaved by multiple enzymes. The caspase most often associated with the given peptide sequence is listed first. † R110 = rhodamine 110; AMC = 7-amino-4-methylcoumarin. The absorption and emission maxima for the cleaved fluorophores at pH 7 are: 496/520 nm for R110 and 342/441 nm for AMC. ‡ EnzChek Caspase-3 Assay Kit. § Bulk packaging. ** RediPlate 96 EnzChek Caspase-3 Assay Kit.|
For Research Use Only. Not for use in diagnostic procedures.