Table 8.1 Specialty nucleic acid reagents for molecular biology.

Cat. No. Dye Ex/Em * Application
Dyes for Ultrasensitive Solution Quantitation †
P7581, P11495, P7589, P11496 Quant-iT PicoGreen dsDNA reagent and Kits 502/523 Ultrasensitive reagent for solution quantitation of dsDNA
O7582, O11492 Quant-iT OliGreen ssDNA reagent and Kit 498/518 Ultrasensitive reagent for solution quantitation of ssDNA and oligonucleotides
R11491, R11490, R32700 Quant-iT RiboGreen RNA reagent and Kits 500/525 Ultrasensitive reagent for solution quantitation of RNA
Dyes for Sensitive Detection of Nucleic Acids in Gels and on Blots
S11494 SYBR Gold nucleic acid gel stain 495/537 Ultrasensitive gel stain for single- or double-stranded DNA or RNA post-electrophoresis

S7563, S7567, S7585

SYBR Green I nucleic acid gel stain 494/521

Ultrasensitive gel stain for double-stranded DNA and oligonucleotides post-electrophoresis; also useful for real-time PCR assays

S7564, S7568, S7586

SYBR Green II RNA gel stain 492/513 Sensitive stain for RNA and single-stranded DNA post-electrophoresis

S33100, S33101, S33102, S33110, S33111, S33112

SYBR Safe DNA stain 502/530 Sensitive DNA gel stain with significantly reduced mutagenicity
* All excitation (Ex) and emission (Em) maxima were determined for dyes bound to double-stranded calf thymus DNA in aqueous solution. † In addition to these dyes, we offer a wide selection of Quant-iT Kits for use with the Qubit fluorometer and with microplate readers; these are listed in Selection guide for the Quant-iT Assay Kits—Table 8.8.