Pathway Focus: Adipokines In Inflammation and Metabolic Diseases (Obesity)
- ABfinity™ rabbit recombinant antibodies are screened to be sensitive and specific
- Enable consistent performance for your assays
- Consistent product from lot-to-lot
ABfinity™ recombinant rabbit antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. Lot-to-lot variability, commonly a problem with polyclonal products, is minimized with ABfinity™ antibodies. In addition to the monoclonal versions of our ABfinity™ antibodies, we offer oligoclonal versions of several of these specificities. Oligoclonal ABfinity™ products are a mixture of recombinant monoclonal antibodies and offer the improved signal strength you get with polyclonal antibodies, combined with the highly reproducible results you get from monoclonals. ABfinity™ antibodies are available to many targets including TNF-α (Gene ID 7124, 21926).
TNF-α (tumor necrosis factor-alpha) is a proinflammatory cytokine that belongs to the tumor necrosis factor superfamily. TNF-α is involved in the regulation of a wide range of biological processes which includes cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Studies have shown that the over expression proinflammatory cytokines is the link between inflammation and obesity. It has been suggested that TNF-α induces adipocyte apoptosis, and promotes insulin resistance by the inhibition of the insulin receptor substrate 1 signaling pathway (Emanuela F et al. (2012) J Nutr Metab, PMCID: PMC3317136).
|Figure 1. Western blot analysis of TNF-α ABfinity™ Recombinant Rabbit Oligoclonal Antibody. Western blot analysis was performed using recombinant TNF-α protein. TNF- α protein (~18 kDa) was detected using TNF- α ABfinity™ Recombinant Rabbit Oligoclonal Antibody (Cat. No. 710288) at 2 µg/mL. The blot was developed using chemiluminescence (ECL) method.|
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
- Quantitative, reliable measurement of single or multiple analytes
- Compatible for use with serum, plasma, and tissue culture supernatant fluids
- Easy-to-run 96-well Novex® assays—4 hours of incubation time
Adipocytes or fat cells are known to release a variety of factors called adipokines. Adipokines and related cytokines play a significant role in the regulation of metabolic processes. These proteins are attracting significant attention as researchers seek to understand their role in obesity and obesity related pathophysiological conditions. Several key adipokines, including resistin, serum amyloid A (SAA), leptin, insulin, lipocalin-2, TNF-α, and IL-6, have been associated with type 2 diabetes, dysfunctional lipid metabolism, and atherosclerosis. Adiponectin, another adipokine, has been inversely correlated with obesity.
We offer single and multiplex Novex® bead-based assays using the Luminex® platform. These assays offer reliable, easy methods to obtain quantitative measurements of adipokines and related cytokines and are suitable for use with the Luminex® 100/200™, FLEXMAP 3D®, and MAGPIX® systems.
- Learn more about Novex® Luminex® Assays
Figure 2. Novex® Luminex® Assay performance. Representative standard curves show the dynamic range and sensitivity of selected adipokines and related cytokines using Novex® bead-based assays. The analysis of assays were conducted using the Luminex® 200™ system.
For Research Use Only. Not for use in diagnostic procedures.
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