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Measure Human β-amyloid Proteins for Alzheimer’s Disease Research

  • Simple—easy-to-run sandwich ELISA
  • Rapid—4-hour incubation protocol and same-day results
  • Convenient—precoated, removable 8-well strips

Alzheimer’s disease (AD) is characterized by the presence of intracellular neurofibrillary tangles and extracellular plaques in the brain, and the major component of these plaques is beta-amyloid peptide (Aβ). Aβ peptide can range from 39 to 43 amino acids in length and is formed after sequential cleavage of the amyloid precursor protein (APP). The predominant forms are Aβ40 and Aβ42, with Aβ40 being the more common of the two and Aβ42 being more associated with disease states.

We offer sandwich ELISA kits to quickly detect and quantify human Aβ in cell culture supernatants, cerebrospinal fluid (CSF), and tissue homogenates for various research models of Alzheimer’s disease. ELISA kits allow results to be collected easily and reproducibly, and calibrated standard curves are provided to accurately quantify the level of protein in each experimental run.



 Figure 1. With a few incubation steps and washes, you can typically have quantitative results in 4 hours.


CSF Dilution
1/4 68.99
1/8 36.90 34.50 107%
1/16 19.43 17.25 113%
1/32 9.09 8.62 105%

Table 1. Measuring Aβ42 in dilutions of cerebrospinal fluid (CSF) using the Aβ42 High Sensitivity ELISA Kit. Human CSF containing Aβ42 at a measured concentration of 280 pg/mL was initially diluted 1:1, then serially diluted in Standard Diluent Buffer over the range of the assay.

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

New ABfinity™ Recombinant Antibodies for Analyzing Neuronal Cells

  • ABfinity™ stem cell recombinant antibodies are screened to be sensitive and specific
  • Consistent performance for your assays
  • Consistent product from lot to lot

ABfinity™ recombinant rabbit antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. Lot-to-lot variability, commonly a problem with polyclonal products, is minimized with ABfinity™ antibodies.

Over the past year we have released several new ABfinity™ recombinant antibodies to support neurobiology research. These antibodies are against a range of markers, including stem cell–related proteins like nucleostemin and musashi-1. In addition to the monoclonal versions, we offer oligoclonal versions of several of these specificities. Oligoclonal ABfinity™ products are a mixture of recombinant monoclonal antibodies and offer the improved signal strength you get with polyclonal antibodies combined with the highly reproducible results you get from monoclonals.


  Figure 2. Immunocytochemistry analysis of HeLa cells stained with (a) Nucleostemin ABfinity™ Recombinant Rabbit Oligoclonal Antibody followed by Alexa Fluor® 488 goat anti–rabbit IgG as secondary, (b) DAPI nuclear stain, (c) Alexa Fluor® 594 phalloidin actin stain. Panel d shows the composite image.

ABfinity™ Stem Cell Antibody Selection Guide

Description Validation* Monoclonal Cat. No. Oligoclonal Cat. No.
Alpha-Synuclein Assay/WB/ICC  701085  710110
Beta Amyloid (1-40) Assay/IHC  --  710174
Beta Amyloid (1-42) Assay/ICC  700254  --
Tau Assay/WB  --  710080
Tau (pS199) Assay/WB  701054  710124
Tau (pT231) Assay/WB  701056  710126
GSK3b (pS9) Assay/WB  701069  710100
Nucleostemin Assay/WB/ICC  701157  710188
PitX3 Assay/WB/ICC  701181  710212
Musashi-1 Assay/WB/ICC  --  710237

*WB = Western blot, ICC = Immunocytochemistry

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

Fluorescent Probes for Neural Tracing and Anatomy

  • Extensive selection of probes for neuronal tracing, anatomy, and function
  • Conjugates of bright and photostable Alexa Fluor® dyes help you get the most information from your samples
  • Proven utility in hundreds of publications

We offer a comprehensive set of Molecular Probes® neural tracing stains. Whether you’re defining neuronal cell connectivity, monitoring translocation through gap junctions, following cells through generational divisions, or identifying specific cells of interest, we have fluorescent and biotinylated probes to help you get the data you’re looking for. All of our Molecular Probes® fluorescent dyes and stains meet stringent quality specifications, and data obtained with them have been featured in hundreds of publications over more than three decades. 


  Figure 3. Coronal section of mouse hippocampus. A 14 µm coronal section of mouse hippocampus stained with an anti–α-tubulin antibody labeled with the Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit. The section was counterstained with the NeuroTrace® 530/615 Red Fluorescent Nissl Stain to visualize neuronal cell bodies, and Hoechst 33258 (Cat. No. H1398, H3569, H21491) to stain nuclei.

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

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