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New P-Selectin ABfinity™ Recombinant Antibodies for Studying Adhesion

  • ABfinity™ P-selectin rabbit recombinant monoclonal and oligoclonal antibodies for sensitive detection
  • Designed for consistent performance for your assays
  • Consistent product from lot to lot

ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. ABfinity™ antibodies are available to many targets, including P-selectin (gene ID 6403).

P-selectin, a cell adhesion molecule found on the surface of endothelial cells, plays a fundamental role in inflammation by bringing leukocytes to the site of injury. P-selectin is rapidly brought to the cell surface after exposure to thrombin and histamine. It has also been found that P-selectin contributes to platelet aggregation in vascular injury.

  Figure 1. Immunocytochemistry analysis of U2OS cells. Cells were stained with P-Selectin ABfinity™ Recombinant Rabbit Monoclonal Antibody using Alexa Fluor® 488 goat anti-rabbit as the secondary antibody (green) (a). DAPI was used to stain the nuclei (blue) (b), and Alexa Fluor® 594 phalloidin was used to stain actin (red) (c). The composite image (d) shows membrane localization of P-selectin.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.

Focus on the Tight Spaces

  • Improved signal-to-noise in immunostaining assays
  • Easy protocol—apply ready-made enhancer solution from a dropper bottle
  • Effective with many fluorescent labels

Image-iT® FX Signal Enhancer is highly effective in blocking background staining that results from nonspecific interactions of a wide variety of fluorescent dyes with cell and tissue constituents. Background staining seen with fluorescent conjugates of streptavidin and antibody conjugates are largely eliminated when Image-iT® FX Signal Enhancer is applied to fixed and permeabilized cells prior to staining.

Resolving the fine details and structures of tightly packed cells and adhesion spots is technically challenging. Some samples can produce unexpected background problems due to nonspecifically adsorbing stains and antibody conjugates. Standard blocking protocols are often insufficient to overcome these challenges. While not always critical, it can be a significant problem when high signal-to-noise is required or when pushing the limits of sensitivity in detecting low-abundance targets. Image-iT® FX Signal Enhancer can significantly reduce this background binding, resulting in marked improvements in staining.
Image-iT® FX Signal Enhancer is easy to use—simply apply the enhancer directly to slides or coverslips containing cell or tissue samples prior to immunostaining. By using Image-iT® FX Signal Enhancer, researchers can get closer to the true signal in their samples and get more reliable results as well as more striking images for publications.



  Figure 2. Golgi complex in fixed and permeabilized HeLa cells labeled with anti–golgin-97 and visualized with green-fluorescent Alexa Fluor® 488 goat-anti–mouse IgG. Actin was stained with red-fluorescent Alexa Fluor® 594 phalloidin; nuclei were stained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). Treatment with Image-iT® FX Signal Enhancer largely eliminates nonspecific dye binding (bottom) as compared to the untreated slide (top).

For Research Use Only. Not for use in diagnostic procedures.

Measure Adhesion Molecules ICAM-1 and VCAM-1 Using ELISA

  • Fast—3 hours or less of incubation time
  • Convenient—ready-to-use ELISA kits
  • Reliable—get consistent, quantitative results

Intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are part of the immunoglobulin superfamily comprising a group of cell surface and soluble proteins involved in recognition, binding, or adhesion processes of cells. These adhesion molecules can be induced by cytokines such as IL-1 and TNF-α.

ICAM-1 and VCAM-1 may play roles in inflammation, cancer, and atherosclerosis. For instance, some melanoma cells can use VCAM-1 to adhere to the endothelium and initiate monocyte recruitment to atherosclerotic sites; this finding has led some researchers to investigate VCAM-1 as a potential therapeutic target.

The ELISA kits are reliable and allow researchers to easily obtain quantitative measurements of adhesion proteins. The assay procedure can be completed within a day in a 96-well plate format. In addition, the strip-well plate format provides the flexibility to run as few samples as needed, allowing you to conserve precious samples.



For Research Use Only. Not for use in diagnostic procedures.

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