Pathway Focus: Metabolic Disorders
||Measuring Acute-Phase Proteins in Metabolic Research—Sandwich ELISA Kits|
|Accurate Detection of Cholesterol in Your Research Samples—The Amplex® Red Cholesterol Assay Kit
|Akt Kinase Signaling Cascade in Type 1 Diabetes—Antibodies for Metabolic Research|
- Quickly detect specific proteins
- Easy-to-use assay
- Reproducible results
Metabolic syndrome is a combination of medical disorders that increase the risk of developing cardiovascular disease and diabetes. Serum amyloid A (SAA), c-reactive protein (CRP), and plasminogen activator inhibitor-1 (PAI-1) play important roles in these diseases. SAA and CRP are acute-phase proteins with plasma concentrations that increase in response to inflammation; inflammatory cells secrete a variety of cytokines into the bloodstream, including IL-6 and TNFα.
We offer sandwich ELISA kits to quickly detect and quantify specific proteins to support your normal and diseased model research. The ELISA kits allow results to be collected in an easy and reproducible fashion. Calibrated standard curves are provided to help accurately quantify the level of protein in each experimental run. ELISA technology allows a more detailed understanding of protein levels in metabolic disorders.
- Simple—detect cholesterol in research samples; no processing required
- Versatile—use with fluorescence or colorimetric detection
- Sensitive—detect as little 80 ng/mL (200 nM)
The Amplex® Red Cholesterol Assay Kit provides a very simple and sensitive fluorometric method to permit accurate quantitation of cholesterol in research samples using a fluorescence microplate reader or fluorometer. Because a large portion of cholesterol in blood is in the form of cholesteryl esters, the assay is based on an enzyme-coupled reaction that detects both free cholesterol and cholesteryl esters.
- Learn more about the Amplex Red Cholesterol Assay Kit
|Figure 2. Detection of cholesterol using the Amplex® Red Cholesterol Assay Kit.
Read More +
Each reaction contained 150 µM Amplex® Red reagent, 1 U/mL HRP, 1 U/mL cholesterol oxidase, 1 U/mL cholesterol esterase, and the indicated amount of cholesterol in 1X reaction buffer. Reactions were incubated at 37°C for 30 min. Fluorescence was measured with a fluorescence microplate reader using excitation at 560 ± 10 nm and fluorescence detection at 590 ± 10 nm. The inset shows the high sensitivity and excellent linearity of the assay at low levels of cholesterol (0–0.015 µg/mL).
- Measure total and phosphorylated proteins
- Highly sensitive and specific antibodies
- Excellent lot-to-lot consistency with ABfinity™ technology
Akt/protein kinase B (Akt) and its signaling cascade are involved in a number of different cellular functions, including glucose uptake, glycogen synthesis, cell growth, survival, apoptosis, protein synthesis, and endothelial nitric oxide production. In Type 1 diabetes, the kidney is exposed to fluctuating levels of blood glucose and exogenous insulin, which is administered as the major treatment of the disease. It has been well established that both glucose and insulin can modulate Akt activity in various cell types and tissues, and thus can affect the signaling cascade and subsequent cellular processes.
Easily Find More Antibodies
|Figure 3. Western blot and peptide competition. Extracts of NIH3T3 cells unstimulated (lane 1) or stimulated with 50 ng/mL PDGF for 5 min (lanes 2–5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.
Read More +
The membrane was blocked with a 5% BSA-TBST buffer for 1 hr at room temperature, then incubated with the Akt/PKB [pS473] rabbit monoclonal antibody for 2 hr at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (lanes 1 and 2), the nonphosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or the phosphopeptide immunogen (lane 5). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP-conjugate and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to Akt/PKB [pS473] completely blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of Akt/PKB [pS473] phosphorylation by the addition of PDGF in this cell system.
|RANBP3 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified
|CD117 (c-Kit)[pY703]ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified
|MEK2 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified
|SAA1 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified
|Hexokinase 2 ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified
|AKT [pS473] ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified
ICC = immunocytochemistry. IF = immunofluorescence. WB = western blot.