Pathway Focus: The Integrin Pathway
- Sensitive assays—allow for detection of low protein levels
- Fast and easy—4-hour incubation protocol
- Improved data—allow generation of quantitative data
FAK is under investigation in several areas of research, including studies of cell adhesion and migration, growth factor–mediated migration, differentiation, cell cycle, apoptosis, and cancer. FAK is overexpressed in breast, colon, and thyroid cancers, possibly contributing to some features of the malignant phenotype (i.e., increased migration and invasion of surrounding stroma).
Our phosphoELISA™ kits offer the ability to quickly measure signaling proteins in cell lysates using a convenient 96-well format. Just process your samples as you would for a western blot, then add them to an ELISA plate, run the assay, and get quantitative results in as little as 4 hours.
- Learn More About phosphoELISA™ Kits
|The specificity of the FAK phosphoELISA™ assay for phosphorylated FAK [pY397] was confirmed by peptide competition.
Only the phosphopeptide containing the phosphorylated tyrosine 397 could block the ELISA signal. The nonphosphorylated peptide sequence or other phosphopeptides from the FAK sequence did not block the signal.
|FAK [pY397] ELISA KIT
|FAK (TOTAL) ELISA KIT
- ABfinity™ p53 [pS15] rabbit recombinant monoclonal antibody for sensitive detection
- Designed for consistent performance for your assays
- Consistent product from lot to lot
ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. ABfinity™ antibodies are available for many targets, including p53 (gene ID 23491729).
- Learn More About ABfinity™ Recombinant Rabbit Monoclonal Antibodies
|p53 [pS15] ABfinity™ Recombinant Rabbit Monoclonal Antibody, purified
|Mouse anti-p53, unconjugated monoclonal
|Mouse anti-p53, unconjugated monoclonal
|p53 [pS392] Rabbit Polyclonal Antibody, unconjugated
||Rat, mouse, human
|Mouse anti-p53, unconjugated monoclonal||Mouse
|Mouse anti-p53, unconjugated monoclonal||Bovine, human
|Alexa Fluor® 488 Goat Anti-Rabbit||Rabbit
- High biological activity
- High purity
- Proven compatibility with Gibco® media
Gibco® recombinant proteins are useful for a wide variety of pathway research investigations. Gibco® recombinant proteins have been bioassayed in cells cultured in Gibco® media. So if you’re already using Gibco® media, you can have more confidence in the compatibility of these reagents in your cell culture–based assays.
Fibronectin plays a role in cell adhesion, growth, migration, and differentiation and is involved in diverse processes impacting wound healing, development, cancer, and fibrosis.
Gibco® cytokines, growth factors, and proteins are high-purity recombinant proteins with high bioactivity. To help ensure Gibco® proteins are of the highest quality, each protein is analyzed for purity along with structural homogeneity so that a biologically active protein is produced.
- Learn More About Gibco® Recombinant Proteins
|Vitronectin Natural Human
|Fibronectin Natural Human
- Broad dynamic range—0.06 to 6,000 pmol (4–5 logs)
- Flexible—can be used in manual or automated, high-throughput formats
- Simple—sample dilution not necessary
Our competitive, heterogeneous cAMP-Screen® and cAMP-Screen Direct® immunoassays measure cAMP directly from cell lysates at levels as low as 60 fmol to 6 µmol, over 4 to 5 logs, without requiring any sample dilution.
Cyclic adenosine monophosphate (cAMP) is an important second messenger in many signal transduction pathways linking activation of cell surface membrane receptors to intracellular responses and, ultimately, to changes in gene expression.
The sensitivity and simplicity of the cAMP-Screen Direct® assay enables researchers to measure small changes in cAMP levels as they investigate integrin-mediated T cell adhesion.
- Over 10 years of published results
- More photostable
Through superior performance, Alexa Fluor® dyes have been contributing to illuminating research for over 10 years. And to help with experiment planning, Alexa Fluor® dyes are supported by an experienced technical support team, >30,000 published references, application and experimental tips, and protocols.
Integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals for cell shape, mobility, and
Because of their superior brightness and photostability, Alexa Fluor® conjugates are superior to most conventional fluorescent secondary reagents and are the detection reagents of choice for many fluorescence-based immunoassays.
- Browse Secondary Antibodies Using Our Secondary Antibody Selector Tool
|Two-color confocal image of a human epidermal whole mount.
ß1 integrin was visualized with the monoclonal antibody P5D2 labeled with the green-fluorescent Alexa Fluor® 488 dye using the Alexa Fluor® 488 Monoclonal Antibody Labeling Kit. a6 integrin was labeled using a mouse monoclonal antibody visualized with Alexa Fluor® 594 goat anti–mouse IgG antibody and pseudocolored blue. Image contributed by Uffe Birk Jensen, University of Aarhus, Denmark.
|Alexa Fluor® 594 goat anti–mouse IgG (H+L), 2 mg/mL
|Alexa Fluor® 488 Monoclonal Antibody Labeling Kit, 5 labelings||1 kit