- Read the latest issue of the BioPath Online E-Newsletter.
Pathway Focus: Metabolic Disorders
- ABfinity™ AS160 [pT642] rabbit recombinant monoclonal and oligoclonal antibodies for sensitive detection
- Designed for consistent performance for your assays
- Consistent product from lot to lot
ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. ABfinity™ antibodies are available against many targets, including AS160 and AMPK alpha.
The AKT substrate AS160 (gene ID 9882) is implicated in signaling cascades that contribute to insulin resistance (especially in individuals with elevated levels of inflammatory markers and increased metabolic risk factors) by inhibiting insulin signaling protein
phosphorylation. When AS160 is phosphorylated at threonine 642 in the presence of higher HDL levels, insulin resistance is reduced. Glucose intake and exercise affect phosphorylation of AS160 T642 and AMPK alpha. T172, and these rapidly changing levels of phosphorylation contribute to many downstream signaling pathways, implicating these critical phosphorylation sites in the understanding of insulin resistance and type 2 diabetes. ABfinity™ recombinant monoclonal antibodies are ideal reagents for signaling pathway research. The ABfinity™ technology helps to ensure consistent antibody performance so you don’t have to reoptimize your assay with each new lot.
- See all ABfinity™ Recombinant Rabbit Monoclonal Antibodies
- Find the Right Antibody With Our Primary Antibodies Search Tool
Figure 1. AS160 [pT642] ABfinity™ Recombinant Rabbit Monoclonal Antibody recognizes a ~160 kDa protein in western blot analysis of serum-starved HeLa cells (whole cell extract, 80 µg per lane).
|AS160 [pT642] ABfinity™ Recombinant Rabbit Monoclonal Antibody, purified
|AS160 [pT642] Recombinant Rabbit Oligoclonal Antibody, purified
|AMPK alpha [pT172] Rabbit Oligoclonal Antibody||Human
- Over 10 years of published results
- Choice of human, mouse, rabbit, rat, chicken, goat, guinea pig, hamster, and sheep reactivities
- Highly cross-adsorbed lots available to minimize cross-reactivity
Alexa Fluor® dyes have been illuminating research for over 10 years. In addition to superior performance, these dyes are backed by an experienced, problem-solving technical support team, >30,000 published references, application and experimental tips, and protocols to help with experimental planning.
Examination of the expression patterns of proteins can provide important support to traditional biochemical data. Alexa Fluor® dyes are conjugated to a selection of secondary antibodies and other detection reagents to enable you to observe the distribution of your target protein within cells or in various tissues. Alexa Fluor® dyes provide a time-tested solution to your imaging needs.
Possessing good brightness and photostability properties, Alexa Fluor® conjugates are superior to most conventional fluorescent secondary reagents and are the detection reagents of choice for use with many fluorescence-based immunoassays.
- See All Available Options With the Secondary Antibody Selector Tool
Figure 2. Mouse intestine cryosection showing basement membranes labeled with chicken IgY anti-fibronectin primary antibody and Alexa Fluor® 647 goat anti–chicken IgG secondary antibody (pseudocolored purple).
- Accurately measure hormones circulating in blood
- Gold-standard, trusted ELISA method
- Rapid, easy-to-perform assays
Insulin, leptin, and adiponectin are polypeptide hormones involved in metabolic processes. Adipocytes express a variety of adipocytokines that function in the homeostatic control of glucose and lipid metabolism. Insulin, released into the blood by the beta cells of the islets of Langerhans in the pancreas, regulates secretion of many of these adipocytokines in response to changes in energy balance.
Typically after eating, insulin is released in response to rising levels of blood glucose.
High glucose levels (occurring either because the body does not produce enough insulin or because cells do not respond to insulin) define diabetes. Adiponectin is exclusively secreted from adipose tissue into the blood and is very abundant in plasma relative to other hormones. Levels of adiponectin are lower in diabetics than in nondiabetics, and weight reduction significantly increases circulating levels. Leptin has an effect similar to that of adiponectin, but the two hormones perform complementary actions and can have additive effects.
By providing accurate and reliable methods to measure hormone levels in research samples, these assay kits are useful research tools that may allow researchers to better understand diseases.
- Learn More About Invitrogen™ ELISA Kits
|Hu Insulin ELISA Kit
|Hu Leptin ELISA Kit
|Ms Leptin ELISA Kit
|Hu Adiponectin ELISA Kit||Human
|Ms Adiponectin ELISA Kit||Mouse
|Rt Adiponectin ELISA Kit||Rat
- Ideal immunoassay tool—simultaneous analysis of up to 50 proteins in limited volume of precious sample
- Excellent performance—accurate, reproducible, and sensitive quantification of proteins
- Reduces time and cost—better approach than multiple, single-analyte ELISAs or western blots
- Easy to use—designed to work with broad and expanding range of Invitrogen™ assay kits
Enzyme-linked immunosorbent assays (ELISAs) have become the standard method for the quantitation of selected protein targets in biological samples. However, ELISAs have been found to be less than ideal for analyzing multiple proteins in single samples.
The MAGPIX® System is built on the well-known Luminex® xMAP® technology, one of the most popular and effective multiplexing technologies for simultaneous measurement of multiple biomarkers. This instrument provides an inexpensive benchtop immunoassay system for performing up to 50 assays on a single biological sample. The MAGPIX® System offers fast, accurate, and easily reproducible results, to help save time and money and preserve valuable samples. This versatile multiplexing platform can be used across a broad range of sample types and protein targets, making it applicable to multiple research settings.
- Learn More About the MAGPIX® System
Figure 4. The MAGPIX® System (A). Standard curve for quantitation of human TNF-alpha in a tissue culture supernatant sample, measured with ELISA and an xMAP® assay. In this singleplex format, the xMAP® assay had excellent correlation with the ELISA (R² = 0.9443) (B).