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Featured article

A sensitive assay for hyperploidy and cell death in the Drosophila brain using the Attune Acoustic Focusing Cytometer

Alzheimer’s disease (AD), characterized by a progressive loss of cognitive function, is the most prevalent neurodegenerative disorder of aging. Cell cycle re-entry and the resulting hyperploidy could be a proximal cause of age-related neurodegeneration, as well as a useful preclinical marker. Dissecting the precise biological significance of cell cycle re-entry in age-related neural decline will be critical to developing new approaches to combat AD.

Fluorophore selection guide for flow cytometry poster

This selection guide includes fluorophores used to study cell viability, cell cycle, cell proliferation, apoptosis, and phagocytosis, as well as fluorescent proteins commonly used in flow cytometry applications. For each dye or fluorescent protein, the excitation laser and common emission filters are shown. Use this poster to choose the best labeling reagents for your next flow cytometry experiment.

Download the poster ›

bp71-flow-cytometry-fluorophore-selection-poster
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Featured new products

Just released
Our newest cell and molecular biology products and technologies

Current research

A sensitive assay for hyperploidy and cell death in the Drosophila brain using the Attune™ Acoustic Focusing Cytometer

Isolation and characterization of exosomes using magnetic beads

Literature review

Few and far between
Tools and strategies for rare-event detection using flow cytometry

Practical applications

The next generation in acoustic focusing cytometry
The Attune™ NxT Acoustic Focusing Cytometer

Flow cytometry panel design: The basics
Multiplex flow cytometry experiments need the right combination of fluorophores

Keep pace with the surge in fluorescent protein choices
Multicolor detection of fluorescent protein expression with the Attune™ NxT Acoustic Focusing Cytometer

Minimize—even eliminate—color compensation
No-compensation immunophenotyping using a four-laser flow cytometer

First, do no harm
No-lyse, no-wash assays for the Attune™ NxT Acoustic Focusing Cytometer

Journal club

Multiparameter detection of early apoptosis markers—Uncompensated polychromatic analysis of mitochondrial membrane potential using JC-1 and multilaser excitation

Online and on the move

 

SpectraViewer mobile app: Now compatible with Android™ and iPhone™ devices

The Molecular Probes™ Fluorescence SpectraViewer mobile app is now available for Android™ phones, and we have updated our iPhone™ and iPad™ version to be compatible with iOS™ 8. This mobile SpectraViewer acts as an extension of our online Fluorescence SpectraViewer, in which you can:

  • View and compare the spectra of fluorescent dyes and proteins
  • Add unlimited emission and excitation filters per plot
  • Email plots and configurations to yourself or your colleagues in a clear, printable format

With a mobile-friendly display and user interface, the SpectraViewer app allows you to search an extensive list of fluorophores and check spectral compatibility when at your lab bench or while you are out and about. Download the Molecular Probes™ Fluorescence SpectraViewer mobile app today:
 

Molecular Probes™ Flow Cytometry Products and Resource Guide

The Molecular Probes™ Flow Cytometry Products and Resource Guide provides an overview of the products and techniques used in flow cytometry workflows, including:

  • Sample preparation—from cell preservation to cell isolation
  • Instrument setup and calibration—from alignment to absolute cell counting
  • Antigen detection—from primary antibodies to custom services
  • Cell analysis—from cell health and viability to phagocytosis

Request your copy—in print or digital format—of the Molecular Probes™ Flow Cytometry Products and Resource Guide
 

Molecular Probes™ flow cytometry mobile app: Protocols at your fingertips

The Molecular Probes™ Flow Cytometry Reagent Guide and Protocols mobile app is designed to help you find fluorescent reagents, kits, and flow cytometry protocols for your cell biology research. Also included are product guides for instrument calibration and setup, protein labeling, and secondary detection. Key features of the app include:

  • Straightforward experimental design, with excitation and emission data for every reagent
  • Quick and easy protocols, with a built-in protocol timer that runs even if you leave the app
  • Examples of data output for every product

Download the Molecular Probes® Flow Cytometry Reagent Guide and Protocols mobile app today:
 

Online cell analysis protocols: Now searchable and sortable

The new “Cell Analysis Protocols” page organizes our staining protocols by platform: flow cytometry, high-content analysis, imaging, and microplate assays; and by application: cell viability, cell proliferation, and cell structure. Simply select the arrow at the top of the column to sort the table for your convenience, or search for the fluorescent probe you are interested in. You’ll find protocols for:

  • Cell viability assays with LIVE/DEAD™ reagents
  • Cell proliferation assays with CellTrace™ reagents
  • Nucleic acid staining with SYTO™ and SYTOX™ dyes

And more! Visit the new Cell Analysis Protocols page
 

Molecular Probes™ School of Fluorescence

Learn the fundamentals of fluorescence imaging from Molecular Probes scientists. We’ve spent hundreds of hours in the lab and want to share our best tips, tricks, and protocols for capturing beautiful images, including:

  • Fundamentals of fluorescence microscopy
  • Sample and fluorescent label considerations
  • Capturing and analyzing your labeled samples
  • Live- and fixed-cell staining protocols

It’s our aim to include everything that we wish we had known when we started working with fluorescent imaging reagents, and we asked our Technical Support team to review each section to make sure we haven’t overlooked anything. Even though we can’t be right there in the lab with you when you do your experiments, we hope you’ll benefit from the information we’ve collected.

For Research Use Only. Not for use in diagnostic procedures.