BioProbes 72—Special Imaging Issue
The Current Issue
Breathe new life into hypoxia research—Intracellular detection of low oxygen tension in live cells
The significance of hypoxia in biological processes is well known; however, creating model systems to accurately control hypoxic conditions is extremely difficult without access to elaborate instruments that allow control and maintenance of temperature, humidity, and gases (O2 and CO2) during an experiment. The EVOS™ FL Auto Imaging System with Onstage Incubator provides an easy-to-use platform that allows precise control of oxygen levels, enabling researchers to evaluate cellular responses to hypoxia using the Image-iT™ Hypoxia Reagent and other fluorescent probes.
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Featured new products
Our newest cell analysis products and technologies
Breathe new life into hypoxia research
Intracellular detection of low oxygen tension in live cells
A key component of an imaging core facility
The EVOS FL Cell Imaging System
Optimize nanoscale imaging at every step
Practical considerations for super-resolution microscopy
At long last, protection from photobleaching for live cells
ProLong Live Antifade Reagent protects fluorescent proteins and dyes in live-cell imaging
Superior protection from photobleaching for fixed cells
ProLong Diamond and SlowFade Diamond Antifade Mountants for fixed-cell imaging
Accessible technology for high-content analysis
Cellinsight CX7 High Content Analysis Platform
Cell health assays
Mimic life in three dimensions
Formation of uniform and reproducible 3D cancer spheroids in high-throughput plates
Tools for studying antibody internalization and trafficking
Applications for therapeutic monoclonal antibodies
Visualize apoptosis in context
Multiplexable Click-iT Plus TUNEL Assays for in situ apoptosis detection
Incorporate automation into your cell analysis workflows
Fluorescence-based viability assays for the Countess II FL Automated Cell Counter
Multiplex your mitochondrial data
Tools to study mitochondrial morphology and function
High-content analysis applied to autophagy
Automating cell imaging with Thermo Scientific HCA systems
From the hood to the microscope: Revolutionizing cell-based imaging
Visit our Cell Culture Café archives for the interactive webinar “From the hood to the microscope: Revolutionizing cell-based imaging”, which showcases some of the latest advances in cell-based research and imaging. We explore how to obtain superior results through the careful selection of reagents and optimization of your imaging workflow—from growing cells on suitable surfaces through to image capture. Particular attention is given to growing and monitoring cells for imaging, fluorescent labeling of live cells, and critical considerations for live-cell and time-lapse imaging.
In this on-demand webinar presented by Magnus Persmark, PhD, Thermo Fisher Scientific, you will learn how to:
- Choose a suitable imaging culture vessel for studying cell growth and viability using brightfield microscopy
- Label cells to maximize signal-to-noise ratios for fluorescence imaging
- Prepare culture conditions to capture images without losing temporal data for time-lapse imaging
- Achieve hypoxic culture conditions by modifying gases or employing multicell spheroid cultures
Get started today at Cell Culture Café
Thermo Fisher Cloud for cell imaging applications
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from one of our cloud-compatible instruments—including EVOS™ Imaging Systems, Cellinsight™ HCA Platforms, and ArrayScan™ HCS Readers—or upload your own files from anywhere you have web access.
With the Cloud Connect Utility, you can upload files by simply placing them in a folder on your computer that is synced to the Cloud. Use one of our cloud-based apps to analyze your data with the power of cloud computing, then share the results with your colleagues to observe patterns, validate findings, and develop future experiments. And it’s not just for data files: upload your presentation for that upcoming conference, or a great how-to video you created.
Receive 10 GB of free storage today when you access Thermo Fisher Cloud
Life science products your research relies on: Now on thermofisher.com
We have united the reliability and innovation of the Thermo Scientific™, Applied Biosystems™, Invitrogen™, Gibco™, and Ion Torrent™ brands to provide you a comprehensive resource you can trust for all the life science products you depend on every day. In particular, you will now find Molecular Probes™ fluorescence reagents and kits under the Invitrogen™ brand, which includes a wide range of cell and molecular biology products and benchtop instruments designed to work together seamlessly. Your former online home for life science products—lifetechnologies.com—has a new look and address.
Learn more about the new thermofisher.com
Essential Knowledge Briefing: Live-Cell Imaging
Although prepared slides reveal many details about biological specimens, they always leave you with questions: How does this look when it’s alive? How does the organism or molecular component behave in a dynamic natural context? How does it interact with its environment? These questions are at the heart of live-cell imaging. In its most basic form, live-cell imaging allows biologists to examine and analyze live samples. This capability completely changes what can be learned about cell and organismal functions and ultimately determines how that information can be applied in fields as diverse as basic research, biotechnology, and medicine.
Published by Wiley, this Essential Knowledge Briefing (EKB) introduces readers to the general field of live-cell imaging. It explains the fundamental challenges and solutions, describes applications of live-cell imaging through case studies, and forecasts some of the next advances.
Download the Essential Knowledge Briefing: Live-Cell Imaging
Molecular Probes Flow Cytometry Products and Resource Guide
The Molecular Probes™ Flow Cytometry Products and Resource Guide provides an overview of the products and techniques used in flow cytometry workflows, including:
- Sample preparation—from cell preservation to cell isolation
- Instrument setup and calibration—from alignment to absolute cell counting
- Antigen detection—from primary antibodies to custom services
- Cell analysis—from cell health and viability to phagocytosis
Request your copy—in print or digital format—of the Molecular Probes™ Flow Cytometry Products and Resource Guide
Molecular Probes cell imaging app: Protocols at your fingertips
The Molecular Probes™ Cell Imaging Reagent Guide and Protocols mobile app is designed to help you find fluorescent reagents, kits, and protocols for cell biology–related fluorescence microscopy applications. Also included are product guides for instrument calibration and setup, protein labeling, and secondary detection. Key features of the app include:
- Straightforward experimental design, with excitation and emission data for every reagent
- Quick and easy protocols, with a built-in protocol timer that runs even if you leave the app
- Examples of data output for every product
Download the Molecular Probes Cell Imaging Reagent Guide and Protocols mobile app today:
24 Hours of Stem Cells: Global virtual conference
Stay on the leading edge of stem cell research with the third annual 24 Hours of Stem Cells.
- Access leading scientific content on demand
- Expand your research network and enrich your perspective in a global context
- Receive virtual training and certifications
- Log in and experience all of this in comfort, from anywhere
Virtual demonstration of the EVOS FL Auto Imaging System
Explore the innovative features of the EVOS™ FL Auto Imaging System and Onstage Incubator with this interactive virtual demonstration. Spin the unit around and interact with the key features of the system. Experience the simple, intuitive software that allows researchers to perform advanced imaging applications regardless of their skill level. Watch videos of time-lapse images collected by the EVOS FL Auto Imaging System. Choose options from the main menu to learn more about how the EVOS FL Auto Imaging System is optimally designed for:
- Live-cell imaging
- Automated cell counting
- Area scanning
- Image tiling/stitching
- Time-lapse imaging
See for yourself how the EVOS FL Auto Imaging System reduces the complexities of microscopy, allowing you to focus on generating great imaging data. Try our interactive virtual demonstration of the EVOS FL Auto Imaging System
Molecular Probes School of Fluorescence
Learn the fundamentals of fluorescence imaging from Molecular Probes scientists. We’ve spent hundreds of hours in the lab and want to share our best tips, tricks, and protocols for capturing beautiful images, including:
- Fundamentals of fluorescence microscopy
- Sample and fluorescent label considerations
- Analysis of your labeled samples
- Live- and fixed-cell staining protocols
It’s our aim to include everything that we wish we had known when we started working with fluorescent imaging reagents, and we asked our Technical Support team to review each section to make sure we haven’t overlooked anything. Even though we can’t be right there in the lab with you when you do your experiments, we hope you’ll benefit from the information we’ve collected.
For Research Use Only. Not for use in diagnostic procedures.