Our newest cell and molecular biology products and technologies

HCS Studio 3.0 delivers additional tools to high-content analysis users

Newly released Thermo Scientific™ HCS Studio™ 3.0 Cell Analysis Software has even broader utility for assay developers and screeners using Thermo Scientific high-content tools. This newest version provides the ability to rapidly assess the quality of your assay through the software’s assay performance tool. Save time by quickly discovering which features will provide the most robust assay, and prevent oversampling in your screen by adjusting your scan’s stopping criteria based on Z-prime results.

Integrated within the HCS Studio 3.0 software and the Store Data and Image Database is the ability to create plate maps that show the assignment of compounds or controls and their concentrations in individual wells. Take advantage of the convenience of storing and editing plate maps and assigning them to one or more plate scans. Whether you are printing plate maps for your lab notebook or simply saving them to the database, all the information you need to identify your experimental plate conditions is included, such as well type, compound, concentration, cell type, and antibody.

Rapid Z-prime tool to measure assay performance.

Superclonal secondary antibodies provide optimal signal-to-noise ratios for immunodetection

Sensitive immunodetection of antigens in cells and tissues requires brightly fluorescent and specific antibodies that produce minimal background staining. Thermo Scientific™ Superclonal™ secondary antibodies represent a breakthrough in recombinant antibody technology, providing exquisitely sensitive binding to their targets while also showing very low levels of nonspecific staining due to cross-reactivity.

To produce Superclonal secondary antibodies, we employ a proprietary screening and production process that yields mixtures of recombinant goat or rabbit secondary antibodies that are selected for their sensitivity and specificity for the target IgG species. These antibodies bind with the epitope-specific precision of monoclonal antibodies, while also achieving the multi-epitope coverage (e.g., both heavy and light chains of target IgGs) and sensitivity of polyclonal antibodies. By comparison, typical polyclonal secondary antibodies are affinity purified from the serum of immunized animals, resulting in a large, undefined pool of antibodies with an unknown set of epitope-binding characteristics. Although broad epitope coverage is a benefit of traditionally produced polyclonal secondary antibodies, poor lot-to-lot consistency due to animal variability and purification processes can lead to cross-reactivity and high background signals.

Superclonal secondary antibodies enable precise and accurate detection of mouse, rabbit, and goat IgG antibodies in cell imaging, ELISA, and western blot applications, with very little cross-reactivity to other species. Their recombinant origin helps ensure lot-to-lot consistency, minimizing the need to optimize each lot before using them in your assays. Superclonal secondary antibodies are available unconjugated, as well as conjugated with biotin, HRP, and selected Alexa Fluor™ dyes.

Reduction in nonspecific staining with Superclonal secondary antibodies. HeLa cell nucleoli were labeled with anti-nucleostemin primary antibody, which was then detected with (A, C) the Alexa Fluor™ 488 conjugate of highly cross-adsorbed goat anti–mouse IgG (H+L) antibody (green), or (B, D) the Alexa Fluor™ 488 conjugate of goat anti–mouse IgG (H+L) Superclonal™ antibody (green). Multicolor images also show cell nuclei stained with DAPI (blue) and actin filaments with Alexa Fluor 594 phalloidin (red). Superclonal secondary antibodies exhibit significantly less cytoplasmic staining, indicating enhanced specificity.