image of BioProbes 73 cover

Featured article

Optimize immunodetection with recombinant secondary antibodies— Superclonal secondary antibodies for sensitive and reproducible immunoassays
Sensitive immunodetection in cells and tissues requires high-affinity antibodies that exhibit minimal background staining. Invitrogen Superclonal secondary antibodies represent a breakthrough in recombinant antibody technology, providing sensitive binding to their targets and very low levels of nonspecific staining. Because of their consistent performance from lot to lot, these next-generation secondary antibodies are becoming the reagent of choice in both research and clinical applications.

The Illuminated Cell poster

The Illuminated Cell poster in the center of BioProbes 73 highlights organelle-specific probes for 14 different cell structures, including the nucleus, endoplasmic reticulum, golgi complex, cytoskeleton, plasma membrane, and mitochondria. Use this poster to choose the best labeling reagents for your next fluorescence imaging experiment.

Download the poster

Featured research tools

Optimize immunodetection with recombinant secondary antibodies
Superclonal secondary antibodies for sensitive and reproducible immunoassays

Protein function and analyses

Cells and soluble mediators of the tumor microenvironment
Antibody-based tools for identifying cell types and proteins of interest

Monitor changes in gene expression using luminescence
The HTS-compatible TurboLuc Luciferase One-Step Glow Assay

Cast your protein gels with ease
Introducing the SureCast Gel Handcast System for protein separation

Load more sample with these precast protein gels
Novex WedgeWell Tris-glycine precast protein gels

Cell viability and proliferation

Multiplex assays for robust cell health analyses
CyQUANT Direct and PrestoBlue viability assays work together

Proliferating and apoptotic cells revealed
Click-iT EdU and Click-iT TUNEL colorimetric assays for immunohistochemistry

Accurately measure cell concentrations by flow cytometry
High-speed cell counting with the Attune NxT Flow Cytometer

Protection from photobleaching for live-cell imaging
ProLong Live Antifade Reagent is here

Tracking cell division with flow cytometry
A guide to studying cell proliferation by generational tracing

Journal club

Appraising the suitability of succinimidyl and lipophilic fluorescent dyes to track proliferation in non-quiescent cells by dye dilution

Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments

In memoriam

A tribute to our colleague Mike Davidson

Cell Analysis Support Center

Introducing our Cell Analysis Support Center

We recently launched our 14th application-specific support center—this one focused on cell analysis. At the Cell Analysis Support Center, you’ll find 12 hubs covering the following topics:

  • Labeling chemistry
  • Microspheres
  • Cell imaging
  • Cell structure and analysis— ICC, IHC, and IF
  • Flow cytometry
  • Microbiological analysis
  • Quantum dots
  • Cell counting
  • Cell viability, proliferation, cryopreservation, and apoptosis
  • Cell tracing and tracking
  • Neuroscience
  • Dynabeads cell isolation and expansion

Additional support centers—including those for protein electrophoresis and western blotting, protein expression, and genome editing—can be found on the Services and Support tab at or at These Support Centers have been created by our technical applications scientists to answer the most commonly asked questions for every step of your experimental workflow, from setting up and running an experiment to analyzing data and troubleshooting.

Visit the Cell Analysis Support Center

LabCoat Live Training Series

LabCoat Live qPCR Training Series: Interactive online lectures with a self-paced lab

The new Applied Biosystems LabCoat Live Training Series is specifically designed to fit into your everyday lab life, whether you are a lab manager who wants help training your staff or a bench scientist conducting research in the lab. LabCoat Live courses simplify complex concepts through a combination of live, online classroom lectures and self-paced lab time over the span of one week. Now you don’t need to take time away from your projects to train others or travel to a training center. LabCoat Live training makes learning new techniques both affordable and convenient with:

  • Two interactive online lectures, providing a thorough understanding of technique, experiment setup, and data analysis so that you can review your results in real time from anywhere with an internet connection
  • An online message board, with step-by-step experiment setup guides to get your questions answered quickly
  • Reagents and a protocol provided to conduct the experiment on your own instrument at a time that works for you

Specifically, the “Introduction to qPCR” module focuses on qPCR fundamentals, applications, and data analysis, including how to set up and perform an absolute quantitation experiment and then analyze standard curve data using Applied Biosystems software.

Register today (not available in all regions) for an upcoming class

Thermo Fisher Scientific mobile apps

Updated mobile apps move to Thermo Fisher Scientific

All of the former Life Technologies mobile apps (both on Google Play™ and at iTunes™) now live under Thermo Fisher Scientific, including all of the Molecular Probes educational apps, as well as those associated with Invitrogen and Applied Biosystems brands. Check out the new look and feel of these updated mobile apps for cell analysis:

  • 3D Cell
  • Cell Imaging Reagent Guide & Protocols
  • Flow Cytometry Reagent Guide & Protocols
  • Fluorescence SpectraViewer

Additionally, all of the iTunes apps have been upgraded to iOS 9.

Download these updated mobile apps today:

And see our complete selection of mobile and desktop apps

Cell Culture Basics virtual training lab

Virtual training labs: Cell culture basics and more

Cell culture is critical for cell biology research, and your experiments rely on your ability to produce and maintain consistently healthy cells. The Cell Culture Basics virtual training lab is an introduction to cell culture that covers many topics, including:

  • Basic principles of cell culture
  • Preparing a new cell culture
  • Performing a cell passage
  • Cryopreservation

This free, 3D interactive learning laboratory offers cell culture training modules, best practices for working with your cells, and quizzes to test your understanding. Make sure you are using state-of-the-art products and procedures for your cell culture by registering for our Cell Culture Basics virtual training lab. We offer several other virtual training labs, including those on pluripotent stem cell culture, transfection, and protein expression.

Register today for the Cell Culture Basics virtual training lab

Free handbook series

Free handbook series: Antibody-based tools for biomedical research

Our new handbook series covers topics in these research areas: cancer signaling, tumor inflammation, stem cell research, and neurodegenerative disease research—with particular emphasis on evaluating protein biomarkers. For each topic, we provide:

  • A brief overview of the current state of research
  • Antibody-based resources, including multiplex characterization of critical proteins
  • Access to detailed protocols for a wide range of experimental techniques, including western blotting, immunofluorescence analysis, flow cytometry, enzyme-linked immunosorbent assays (ELISAs), and more

Download one or all of these technical handbooks

For Research Use Only. Not for use in diagnostic procedures.